Enhancement of D-lactic acid production from a mixed glucose and xylose substrate by the Escherichia coli strain JH15 devoid of the glucose effect

BMC Biotechnology
Hongying LuShengde Zhou

Abstract

A thermal tolerant stereo-complex poly-lactic acid (SC-PLA) can be made by mixing Poly-D-lactic acid (PDLA) and poly-L-lactic acid (PLLA) at a defined ratio. This environmentally friendly biodegradable polymer could replace traditional recalcitrant petroleum-based plastics. To achieve this goal, however, it is imperative to produce optically pure lactic acid isomers using a cost-effective substrate such as cellulosic biomass. The roadblock of this process is that: 1) xylose derived from cellulosic biomass is un-fermentable by most lactic acid bacteria; 2) the glucose effect results in delayed and incomplete xylose fermentation. An alternative strain devoid of the glucose effect is needed to co-utilize both glucose and xylose for improved D-lactic acid production using a cellulosic biomass substrate. A previously engineered L-lactic acid Escherichia coli strain, WL204 (ΔfrdBC ΔldhA ΔackA ΔpflB ΔpdhR ::pflBp6-acEF-lpd ΔmgsA ΔadhE, ΔldhA::ldhL), was reengineered for production of D-lactic acid, by replacing the recombinant L-lactate dehydrogenase gene (ldhL) with a D-lactate dehydrogenase gene (ldhA). The glucose effect (catabolite repression) of the resulting strain, JH13, was eliminated by deletion of the ptsG gene which encodes...Continue Reading

References

Apr 2, 1999·Applied and Environmental Microbiology·D E ChangJ G Pan
Feb 26, 2000·Enzyme and Microbial Technology·K Hofvendahl, B Hahn-Hägerdal
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Nov 6, 2001·Applied Microbiology and Biotechnology·V Hernández-MontalvoG Gosset
Oct 31, 2002·Journal of Industrial Microbiology & Biotechnology·B S DienR J Bothast
Jul 11, 2006·Applied Microbiology and Biotechnology·Yutaka Tokiwa, Buenaventurada P Calabia
Nov 24, 2006·Applied and Environmental Microbiology·Y ZhuE Altman
Jan 17, 2007·Applied Microbiology and Biotechnology·Rojan P JohnAshok Pandey
Jan 12, 2008·Applied Microbiology and Biotechnology·Shohei OkinoHideaki Yukawa
May 28, 2009·Journal of Industrial Microbiology & Biotechnology·José UtrillaAlfredo Martinez
Dec 22, 2009·Applied and Environmental Microbiology·Tasha A Desai, Christopher V Rao
Dec 25, 2009·Biotechnology Letters·D S JoshiD V Gokhale
May 18, 2010·Applied and Environmental Microbiology·Suman MazumdarRamon Gonzalez
Mar 2, 2011·Applied Biochemistry and Biotechnology·Luciana Fontes CoelhoJonas Contiero
Apr 30, 2013·Biotechnology Advances·Mohamed Ali Abdel-RahmanKenji Sonomoto
Jan 22, 2015·Biotechnology Letters·Mark A Eiteman, Subramanian Ramalingam

❮ Previous
Next ❯

Citations

Oct 21, 2016·Applied Microbiology and Biotechnology·Silvia KlotzUlf Prüße
Feb 23, 2020·Biotechnology and Bioengineering·Mohamed Ali Abdel-RahmanKenji Sonomoto
Mar 13, 2020·Biotechnology Journal·Yu Jung SohnSi Jae Park

❮ Previous
Next ❯

Methods Mentioned

BETA
phosphotransferase
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.