Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp

Biotechnology for Biofuels
Xiao HanZhi Chen

Abstract

Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid that has a variety of physiological functions for infant development and human health. Although metabolic engineering was previously demonstrated to be a highly efficient way to rapidly increase lipid production, metabolic engineering has seldom been previously used to increase DHA accumulation in Schizochytrium spp. Here, a sensitive β-galactosidase reporter system was established to screen for strong promoters in Schizochytrium sp. Four constitutive promoters (EF-1α p , TEF-1 p , ccg1 p , and ubiquitin p ) and one methanol-induced AOX1 promoter were characterized by the reporter system with the promoter activity ccg1 p > TEF-1 p  > AOX1 p (induced) > EF-1α p  > ubiquitin p . With the strong constitutive promoter ccg1 p , Schizochytrium ATP-citrate lyase (ACL) and acetyl-CoA carboxylase (ACC) were overexpressed in Schizochytrium sp. ATCC 20888. The cells were cultivated at 28 °C and 250 rpm for 120 h with glucose as the carbon source. Shake-flask fermentation results showed that the overexpression strains exhibited growth curves and biomass similar to those of the wild-type strain. The lipid contents of the wild-type strain and o...Continue Reading

References

Nov 1, 2001·Journal of Industrial Microbiology & Biotechnology·K W FanL L Vrijmoed
Mar 13, 2009·Bioprocess and Biosystems Engineering·Lu-Jing RenXiao-Jun Ji
Feb 12, 2010·The Journal of Nutrition·Robert K McNamara
Aug 2, 2011·Bioresource Technology·ZhaoSheng LiBaoZhen Li
Feb 26, 2014·Journal of Industrial Microbiology & Biotechnology·Hong ChenHao Liu
Dec 9, 2014·Metabolic Engineering·Anastasia KrivoruchkoJens Nielsen
May 31, 2015·Molecular Phylogenetics and Evolution·Ryan M R GawrylukAndrew J Roger
Jul 1, 2015·FEMS Yeast Research·Cipriano Chávez-CabreraLuis Bernardo Flores-Cotera
Oct 24, 2015·Journal of Agricultural and Food Chemistry·Lu-jing RenHe Huang
May 26, 2016·Nature Communications·Yongjin J ZhouJens Nielsen
Jul 19, 2016·Critical Reviews in Biotechnology·Michio HashimotoHiroyuki Arai
Oct 21, 2016·Cell Reports·Steven ZhaoKathryn E Wellen
Mar 16, 2017·Biotechnology and Bioengineering·Peng XuGregory Stephanopoulos
Jul 29, 2017·Clinical and Translational Medicine·Rafael ZárateCovadonga Rodríguez

❮ Previous
Next ❯

Citations

Mar 18, 2021·Biotechnology Advances·Fei DuHe Huang
Mar 28, 2021·The Science of the Total Environment·Ayesha AslamMohammed Zwawi
Nov 18, 2020·Trends in Biotechnology·Xiao-Man SunHe Huang
Nov 16, 2021·Critical Reviews in Biotechnology·Yu-Lei JiaQi Li

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.