Enhancement of drug delivery to bone: characterization of human tissue-nonspecific alkaline phosphatase tagged with an acidic oligopeptide.

Molecular Genetics and Metabolism
Tatsuo NishiokaW S Sly

Abstract

Hypophosphatasia is caused by deficiency of activity of the tissue-nonspecific alkaline phosphatase (TNSALP), resulting in a defect of bone mineralization. Enzyme replacement therapy (ERT) with partially purified plasma enzyme was attempted but with little clinical improvement. Attaining clinical effectiveness with ERT for hypophosphatasia may require delivering functional TNSALP enzyme to bone. We tagged the C-terminal-anchorless TNSALP enzyme with an acidic oligopeptide (a six or eight residue stretch of L-Asp), and compared the biochemical properties of the purified tagged and untagged enzymes derived from Chinese hamster ovary cell lines. The specific activities of the purified enzymes tagged with the acidic oligopeptide were the same as the untagged enzyme. In vitro affinity experiments showed the tagged enzymes had 30-fold higher affinity for hydroxyapatite than the untagged enzyme. Lectin affinity chromatography for carbohydrate structure showed little difference among the three enzymes. Biodistribution pattern from single infusion of the fluorescence-labeled enzymes into mice showed delayed clearance from the plasma up to 18 h post infusion and the amount of tagged enzyme retained in bone was 4-fold greater than that of...Continue Reading

References

May 1, 1975·Acta Medica Scandinavica·E Sorensen, H Flodgaard
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P D StahlP H Schlesinger
Sep 1, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·R J WaltonJ A Kanis
Jan 1, 1989·Acta Paediatrica Scandinavica. Supplement·M WeningerA Pollak
Jan 1, 1989·Connective Tissue Research·W T Butler
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A OldbergD Heinegård
Jan 1, 1988·Pediatric Pathology·M M SilverK J Milne
Jan 1, 1983·Methods in Enzymology·C R MerrilM L Van Keuren
May 15, 1984·Archives of Biochemistry and Biophysics·J L Meyer
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·R M ShullE F Neufeld
Jun 1, 1994·The Journal of Clinical Investigation·M S SandsE H Birkenmeier
Apr 15, 1996·The Journal of Clinical Investigation·A C CrawleyJ J Hopwood
Mar 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S NarisawaJ L Millán
Mar 1, 1997·Calcified Tissue International·A L BoskeyZ Schwartz
Aug 1, 1997·Journal of Dental Research·A L Boskey
Jan 23, 1998·Journal of Dental Research·L C ChowG L Vogel
May 23, 1998·The International Journal of Biochemistry & Cell Biology·F A LeoneJ M Pizauro
May 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S KasugaiK Ohya
Feb 15, 2001·The New England Journal of Medicine·E D KakkisE F Neufeld
Jul 7, 2001·The New England Journal of Medicine·C M EngUNKNOWN International Collaborative Fabry Disease Study Group
Jan 5, 2002·Journal of Inherited Metabolic Disease·J A Barranger, E O'Rourke
Aug 7, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sonia Di MauroJose Luis Millán
May 1, 1957·The American Journal of Medicine·D FRASER
Jan 1, 1958·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M McGEE-RUSSELL

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Citations

Aug 28, 2013·Journal of Inherited Metabolic Disease·Hirotaka OikawaWilliam S Sly
Mar 25, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shunji TomatsuWilliam S Sly
Jun 20, 2012·Genetic Vaccines and Therapy·Ni-Chung LeeWuh-Liang Hwu
Nov 20, 2008·Journal of Bone and Mineral Metabolism·Junko IshizakiKen-Ichi Miyamoto
Jun 19, 2010·Expert Opinion on Emerging Drugs·Michael Beck
Nov 25, 2014·Expert Opinion on Orphan Drugs·Shunji TomatsuTadao Orii
Feb 3, 2016·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Lesley J Scott
Nov 23, 2015·Calcified Tissue International·José Luis Millán, Michael P Whyte
Oct 22, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tessa LuhmannLorenz Meinel
Dec 19, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·José Luis MillánMichael P Whyte
Aug 6, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Seiko YamamotoTakashi Shimada
Oct 1, 2009·Human Mutation·Tse Siang Kang, Raymond C Stevens
Aug 21, 2012·Molecular Genetics and Metabolism·Daniel J RowanWilliam S Sly
Nov 28, 2013·Archives of Disease in Childhood·Takeshi TaketaniSeiji Yamaguchi
Mar 25, 2008·Molecular Genetics and Metabolism·Adriana M MontañoWilliam S Sly
May 8, 2016·American Journal of Medical Genetics. Part a·Patrick Yap, Ravi Savarirayan
Nov 3, 2016·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tatsuo Takahashi
Mar 6, 2007·Molecular Genetics and Metabolism·Shunji TomatsuTadao Orii
Jun 24, 2017·International Journal of Molecular Sciences·Molly StapletonShunji Tomatsu
Aug 20, 2018·The Journal of Endocrinology·Guillaume MabilleauDaniel Chappard
Jul 30, 2008·Pharmaceutical Research·Tatsuo TakahashiKen-ichi Miyamoto
Aug 28, 2020·Current Osteoporosis Reports·Kazuki SawamotoShunji Tomatsu
Jan 1, 2020·Orphanet Journal of Rare Diseases·Ataf Hussain Sabir, Trevor Cole
Jan 8, 2009·Journal of Orthopaedic Surgery·H W LeungW Y Shen
Mar 23, 2017·Frontiers in Pharmacology·Angie C JelinJulie Hoover-Fong
Apr 26, 2019·Journal of Human Genetics·Caitlin DohertyShunji Tomatsu
Oct 17, 2018·Pediatric Research·Carlos J Alméciga-DíazShunji Tomatsu
Jun 25, 2020·Molecular Therapy. Methods & Clinical Development·Kazuki SawamotoShunji Tomatsu
Jul 13, 2021·Journal of Orthopaedic Translation·Timothy DreyerGill Holdsworth

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