Enhancement of dynamin polymerization and GTPase activity by Arc/Arg3.1

Biochimica Et Biophysica Acta
Christopher ByersJoseph P Albanesi

Abstract

The Activity-regulated cytoskeleton-associated protein, Arc, is an immediate-early gene product implicated in various forms of synaptic plasticity. Arc promotes endocytosis of AMPA type glutamate receptors and regulates cytoskeletal assembly in neuronal dendrites. Its role in endocytosis may be mediated by its reported interaction with dynamin 2, a 100 kDa GTPase that polymerizes around the necks of budding vesicles and catalyzes membrane scission. Enzymatic and turbidity assays are used in this study to monitor effects of Arc on dynamin activity and polymerization. Arc oligomerization is measured using a combination of approaches, including size exclusion chromatography, sedimentation analysis, dynamic light scattering, fluorescence correlation spectroscopy, and electron microscopy. We present evidence that bacterially-expressed His6-Arc facilitates the polymerization of dynamin 2 and stimulates its GTPase activity under physiologic conditions (37°C and 100mM NaCl). At lower ionic strength Arc also stabilizes pre-formed dynamin 2 polymers against GTP-dependent disassembly, thereby prolonging assembly-dependent GTP hydrolysis catalyzed by dynamin 2. Arc also increases the GTPase activity of dynamin 3, an isoform of implicated i...Continue Reading

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Citations

Apr 14, 2016·Neural Plasticity·Sumasri GuntupalliVictor Anggono
Sep 30, 2020·PloS One·Melissa BoldridgeLei Wang
Jul 15, 2015·The Biochemical Journal·Cameron Day, Jason D Shepherd
Jan 30, 2019·Frontiers in Cellular Neuroscience·Francisca Acuña-HinrichsenCarola Otth
Oct 6, 2017·Frontiers in Cellular Neuroscience·Craig MyrumClive R Bramham
May 30, 2017·Frontiers in Synaptic Neuroscience·Rajeevkumar R NairClive R Bramham
Sep 30, 2016·Journal of Psychopharmacology·Pirathiv KugathasanYan Li
Jul 14, 2020·Frontiers in Synaptic Neuroscience·Joseph P AlbanesiDavid M Jameson
Sep 6, 2020·The European Journal of Neuroscience·Hongyu Zhang, Clive R Bramham
Mar 26, 2021·Frontiers in Molecular Biosciences·Per Niklas HeddeDavid M Jameson
Jun 3, 2021·Journal of Neurochemistry·Alicyia Walczyk-MooradallyJasmin Lalonde
Dec 22, 2017·Biochemistry·Barbara BarylkoJoseph P Albanesi

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