Abstract
Lysophosphatidic acid (LPA) receptors belong to G protein-coupled transmembrane receptors (LPA receptors; LPA(1) to LPA(6)). They indicate a variety of cellular response by the interaction with LPA, including cell proliferation, migration and differentiation. Recently, we have reported that constitutive active mutated LPA(1) induced the strong biological effects of rat neuroblastoma B103 cells. In the present study, we examined the effects of mutated LPA(1) on the interaction between B103 cells and endothelial F-2 cells. Each LPA receptor expressing B103 cells were maintained in serum-free DMEM and cell motility assay was performed with a Cell Culture Insert. When F-2 cells were cultured with conditioned medium from Lpar1 and Lpar3-expressing cells, the cell motility of F-2 cells was significantly higher than control cells. Interestingly, the motile activity of F-2 cells was strongly induced by mutated LPA(1) than other cells, correlating with the expression levels of vascular endothelial growth factor (Vegf)-A and Vegf-C. Pretreatment of LPA signaling inhibitors inhibited F-2 cell motility stimulated by mutated LPA(1). These results suggest that activation of LPA signaling via mutated LPA(1) may play an important role in the p...Continue Reading
References
Nov 18, 1971·The New England Journal of Medicine·J Folkman
Apr 29, 1993·Nature·K J KimN Ferrara
Jun 19, 2002·Biochimica Et Biophysica Acta·Xianjun FangGordon B Mills
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Jun 11, 2004·Annual Review of Biochemistry·Isao IshiiJerold Chun
Sep 15, 2006·Biochemical and Biophysical Research Communications·Toshifumi TsujiuchiNobuyuki Fukushima
Dec 26, 2007·Biochimica Et Biophysica Acta·Shinya ShanoNobuyuki Fukushima
Jul 17, 2008·Cellular Signalling·Chi-Iou LinHsinyu Lee
Nov 13, 2008·Mutation Research·Yumi OboToshifumi Tsujiuchi
Nov 13, 2008·Journal of the National Cancer Institute·Shuangxing YuGordon B Mills
Nov 26, 2008·Biochemical and Biophysical Research Communications·Takanori YamadaToshifumi Tsujiuchi
Jan 3, 2009·Current Opinion in Pharmacology·Kyoko NoguchiJerold Chun
Jun 16, 2009·Prostaglandins & Other Lipid Mediators·Satoshi IshiiKeisuke Yanagida
Mar 25, 2010·Prostaglandins & Other Lipid Mediators·Mu-En LinJerold Chun
Oct 5, 2010·Virchows Archiv : an International Journal of Pathology·Megumu TsujinoToshifumi Tsujiuchi
Jan 27, 2011·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Kyoko OkabeToshifumi Tsujiuchi
Nov 15, 2011·Cancer Letters·Mai HayashiToshifumi Tsujiuchi
Dec 27, 2011·Biochemical and Biophysical Research Communications·Kohei KatoToshifumi Tsujiuchi
Mar 1, 2010·Journal of Toxicologic Pathology·Naoko WakabayashiToshifumi Tsujiuchi
Citations
Jul 6, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rie FukuiToshifumi Tsujiuchi
Oct 9, 2012·Experimental Cell Research·Kyohei YoshikawaToshifumi Tsujiuchi
Apr 8, 2015·Biochemical and Biophysical Research Communications·Shuhei IshiiToshifumi Tsujiuchi
May 7, 2014·Growth Factors·Mutsumi ArakiToshifumi Tsujiuchi
Aug 6, 2013·Journal of Receptor and Signal Transduction Research·Eriko TanabeToshifumi Tsujiuchi
Jan 28, 2014·Journal of Receptor and Signal Transduction Research·Toshifumi TsujiuchiNobuyuki Fukushima
Mar 21, 2013·Biochemical and Biophysical Research Communications·Ayano ShibataToshifumi Tsujiuchi
Jun 28, 2015·Molecular and Cellular Biochemistry·Shiori MoriToshifumi Tsujiuchi
Oct 9, 2012·Journal of Cellular Physiology·Filomena de NigrisClaudio Napoli
Oct 15, 2020·Biomedicines·Rohit GundamarajuSukhwinder Singh Sohal