Enhancement of endotoxin neutralization by coupling of a C12-alkyl chain to a lactoferricin-derived peptide

The Biochemical Journal
Jörg AndräKlaus Brandenburg

Abstract

Antibacterial peptide acylation, which mimics the structure of the natural lipopeptide polymyxin B, increases antimicrobial and endotoxin-neutralizing activities. The interaction of the lactoferricin-derived peptide LF11 and its N-terminally acylated analogue, lauryl-LF11, with different chemotypes of bacterial lipopolysaccharide (LPS Re, Ra and smooth S form) was investigated by biophysical means and was related to the peptides' biological activities. Both peptides exhibit high antibacterial activity against the three strains of Salmonella enterica differing in the LPS chemotype. Lauryl-LF11 has one order of magnitude higher activity against Re-type, but activity against Ra- and S-type bacteria is comparable with that of LF11. The alkyl derivative peptide lauryl-LF11 shows a much stronger inhibition of the LPS-induced cytokine induction in human mononuclear cells than LF11. Although peptide-LPS interaction is essentially of electrostatic nature, the lauryl-modified peptide displays a strong hydrophobic component. Such a feature might then explain the fact that saturation of the peptide binding takes place at a much lower peptide/LPS ratio for LF11 than for lauryl-LF11, and that an overcompensation of the negative LPS backbone ...Continue Reading

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Citations

Mar 4, 2011·Applied Microbiology and Biotechnology·Klaus BrandenburgThomas Gutsmann
Apr 11, 2008·Bulletin of Experimental Biology and Medicine·S V ZubovaI R Prokhorenko
Feb 27, 2009·The Journal of Biological Chemistry·Jiri MaresOliver Zerbe
Jul 8, 2010·Antimicrobial Agents and Chemotherapy·Thomas GutsmannKlaus Brandenburg
Feb 25, 2009·Antimicrobial Agents and Chemotherapy·Griselda N SerranoFrank Schweizer
Feb 4, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Daniela LatorrePatrizia Puddu
May 1, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Hans J Vogel
Jan 1, 2009·International Journal of General Medicine·Isabella R ProkhorenkoSergey V Grachev
Aug 10, 2007·Expert Opinion on Investigational Drugs·Roman Jerala
Jan 24, 2016·Biochimica Et Biophysica Acta·Klaus BrandenburgKarl Lohner
Jun 5, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Denise GreathouseTaylor Ladd
Oct 31, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Yong Hai NanSong Yub Shin
Jun 9, 2007·Biochimica Et Biophysica Acta·Xi ChenKlaus Brandenburg
Apr 18, 2006·Biochimica Et Biophysica Acta·Dagmar ZweytickKarl Lohner
Jan 20, 2011·Biophysical Journal·Thomas EtzerodtThomas L Andresen
Feb 3, 2015·International Journal of Peptide Research and Therapeutics·Elżbieta KamyszWojciech Kamysz
Nov 22, 2011·International Immunopharmacology·Maria Elisa Drago-SerranoRafael Campos-Rodríguez
Jun 3, 2015·Antimicrobial Agents and Chemotherapy·Ya-Han ChihJya-Wei Cheng
Oct 4, 2008·ChemMedChem·Carlos Mas-MorunoFernando Albericio
Aug 2, 2006·Molecular BioSystems·Vikrant M BhorAvadhesha Surolia
Apr 20, 2014·The International Journal of Artificial Organs·Stephan HarmJens Hartmann

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