Enhancement of ethanol production in very high gravity fermentation by reducing fermentation-induced oxidative stress in Saccharomyces cerevisiae

Scientific Reports
Thanawat BurphanChoowong Auesukaree

Abstract

During fermentation, yeast cells encounter a number of stresses, including hyperosmolarity, high ethanol concentration, and high temperature. Previous deletome analysis in the yeast Saccharomyces cerevisiae has revealed that SOD1 gene encoding cytosolic Cu/Zn-superoxide dismutase (SOD), a major antioxidant enzyme, was required for tolerances to not only oxidative stress but also other stresses present during fermentation such as osmotic, ethanol, and heat stresses. It is therefore possible that these fermentation-associated stresses may also induce endogenous oxidative stress. In this study, we show that osmotic, ethanol, and heat stresses promoted generation of intracellular reactive oxygen species (ROS) such as superoxide anion in the cytosol through a mitochondria-independent mechanism. Consistent with this finding, cytosolic Cu/Zn-SOD, but not mitochondrial Mn-SOD, was required for protection against oxidative stress induced by these fermentation-associated stresses. Furthermore, supplementation of ROS scavengers such as N-acetyl-L-cysteine (NAC) alleviated oxidative stress induced during very high gravity (VHG) fermentation and enhanced fermentation performance at both normal and high temperatures. In addition, NAC also pl...Continue Reading

References

May 11, 2000·Molecular and Cellular Biology·L T Jensen, V C Culotta
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Aug 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Edward LukValeria Cizewski Culotta
Jul 11, 2006·Biochimica Et Biophysica Acta·Valeria Cizewski CulottaThomas V O'Halloran
Mar 28, 2007·Applied Microbiology and Biotechnology·Xiaoyi Du, Hiroshi Takagi
Jul 25, 2007·FEMS Microbiology Reviews·Brian R GibsonKatherine A Smart
Jan 8, 2008·Biochimica Et Biophysica Acta·Enrique HerreroElisa Cabiscol
Jan 15, 2010·Journal of Applied Microbiology·D StanleyG A Stanley
Jun 23, 2011·Journal of Industrial Microbiology & Biotechnology·Pradeep PuligundlaSanghoon Ko
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mark RinnerthalerMichael Breitenbach
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Jacob VergheseKevin A Morano
Dec 1, 2015·Frontiers in Bioengineering and Biotechnology·Luis CaspetaJens Nielsen
Mar 1, 2016·Frontiers in Microbiology·Elisabet Navarro-TapiaRoberto Pérez-Torrado
Mar 20, 2016·Applied and Environmental Microbiology·Sirikarn CharoenbhakdiChoowong Auesukaree

❮ Previous
Next ❯

Citations

Feb 9, 2020·Applied and Environmental Microbiology·Guoxing ZhuJianrong Wu
Apr 16, 2020·Environmental Microbiology·Todsapol TechoChoowong Auesukaree
Feb 23, 2021·Biotechnology and Bioengineering·Thiago J B MesquitaAntonio C L Horta
May 11, 2021·World Journal of Microbiology & Biotechnology·L Solieri

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
protein folding

Software Mentioned

ImageJ
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.