Enhancement of gene expression efficiency using cationic liposomes on ovarian cancer cells

Drug Delivery
Takehiro SerikawaKenichi Tanaka

Abstract

We performed transfection using cationic liposome. According to the gene expression level, lined cells were divided into two groups, high and low. Introduced gene was monitored with a confocal laser-scanning microscope. The percentages of the cells introduced gene reached more than 90% in all line. Then, introduced gene was stable in high group, while in low group, it significantly decreased. With lysosomotrophic agents, gene expression efficiency was significantly reduced.With colchicine, gene expression efficiency did not change in high group, but was significantly elevated in low group. A method of liposomal transfection could be effective, particularly in low group.

References

Aug 25, 1992·Nucleic Acids Research·Y Ishikawa, C J Homcy
Jan 26, 1989·Nature·P L Felgner, G M Ringold
Aug 11, 1995·The Journal of Biological Chemistry·J ZabnerM J Welsh
Oct 16, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·H KikuchiK Tanaka
Mar 12, 2003·Trends in Biotechnology·Kenneth Lundstrom
Jun 15, 2005·Annual Review of Biochemistry·Inder M Verma, Matthew D Weitzman
Sep 24, 2005·The Journal of Obstetrics and Gynaecology Research·Takaaki SatoKenichi Tanaka
Jun 24, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Takehiro SerikawaKenichi Tanaka
Aug 18, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Luc Wasungu, Dick Hoekstra

❮ Previous
Next ❯

Citations

Aug 11, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Meenu MehtaKamal Dua

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.