PMID: 8993406Dec 26, 1996Paper

Enhancement of glucose-induced insulin secretion in transgenic mice overexpressing human VIP gene in pancreatic beta cells

Annals of the New York Academy of Sciences
I KatoH Okamoto

Abstract

Using transgenic mice technology, it has now become possible to test directly whether VIP and PHM-27 can enhance glucose-induced insulin secretion and reduce blood glucose in vivo. By microinjecting the entire human VIP gene ligated to the rat insulin II promoter, we have established a mouse model that overproduces VIP and PHM-27 in pancreatic beta cells. VIP was secreted from transgenic islets in a glucose-dependent manner. Analyses of these VIP-transgenic mice indicated that the transgene efficiently enhances glucose-induced insulin secretion and significantly reduces blood glucose as compared with control mice. The transgene also ameliorated glucose intolerance of 70% depancreatized mice. The present results suggest that somatic cell gene therapy directed to diabetic islets by human VIP/PHM-27 gene introduction may provide a means to improve the secretory function of the diabetic islets.

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Citations

Jul 28, 2001·Diabetes Technology & Therapeutics·R PerfettiS Busselen
Sep 20, 2012·Journal of Molecular Endocrinology·Ahter D SanliogluSalih Sanlioglu
Mar 21, 2015·International Journal of Radiation Biology·Adnan M Ikhtiar
Oct 30, 1998·Molecular Medicine Today·A W Varley, R S Munford
May 11, 2021·Frontiers in Endocrinology·Erwin Ilegems, Per-Olof Berggren
May 27, 2021·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Anna SemaniakouValerie Chappe

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