Enhancement of human prolactin synthesis by sodium butyrate addition to serum-free CHO cell culture.

Journal of Biomedicine & Biotechnology
H Rodrigues GoulartCarlos R J Soares

Abstract

Sodium butyrate (NaBu) has been used as a productivity enhancer for the synthesis of recombinant proteins in Chinese hamster ovary (CHO) cells. Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL) from CHO cells was investigated for the first time. CHO cell cultures were submitted to a treatment with different concentrations of NaBu (0.25 to 4 mM). Quantitative and qualitative analyses by reverse-phase high-performance liquid chromatography (RP-HPLC) and Western blot or SDS-PAGE, carried out directly on CHO-conditioned medium, showed that the highest hPRL expression was obtained with 1 mM NaBu. In vitro biological assays based on noble rat lymphoma (Nb2) and mouse pro-B lymphoma (Ba/F3-LLP) cells were carried out on purified hPRL. Its bioactivity in the presence of NaBu was not apparently different from that of the First International Reference Reagent of recombinant hPRL (WHO 97/714). Our results show that NaBu increased the synthesis of recombinant hPRL in CHO cells, apparently without compromising either its structure or function.

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Citations

Jun 14, 2012·Applied Biochemistry and Biotechnology·Fernanda Santos ArthusoCarlos Roberto Jorge Soares
Sep 3, 2013·Applied Biochemistry and Biotechnology·Renata DamianiMaria Teresa C P Ribela
May 5, 2021·Biotechnology Advances·Michelle Combe, Stanislav Sokolenko

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Size-Exclusion Chromatography
electrophoresis
size exclusion chromatography

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