Abstract
The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit as biopharmaceutical companies migrate their drug pipelines away from small organic molecules. As such, optimization of monoclonal antibody technologies is important, as this is becoming the new rate-limiting step for discovery and development of new pharmaceuticals. The major limitations of this system are the efficiency of isolating hybridoma clones, the process of stabilizing these clones and optimization of hybridoma cell secretion, especially for large-scale production. Many previous studies have demonstrated how perturbations in the aqueous environment can impact upon cell biology. In particular, radio frequency (RF) irradiation of solutions can have dramatic effects on behavior of solutions, cells and in particular membrane proteins, although this effect decays following removal of the RF. Recently, it was shown that nanoparticle doping of RF irradiated water (NPD water) produced a stabilized aqueous medium that maintained the characteristic properties of RF irradiated water for extended periods of time. Therefore, the ordering effect in water of the RF irradiation can now be studied in systems that required prolonge...Continue Reading
References
Aug 7, 1975·Nature·G Köhler, C Milstein
Jan 21, 1992·Journal of Immunological Methods·R Pannell, C Milstein
Jan 17, 1989·Journal of Immunological Methods·R Bazin, R Lemieux
Jun 19, 1995·FEBS Letters·E E Fesenko, Gluvstein AYa
Jun 5, 1995·FEBS Letters·E E FesenkoN K Chemeris
Jan 13, 1995·Journal of Immunological Methods·R JaspertJ L'age-Stehr
Feb 6, 1995·FEBS Letters·V I GeletyukE E Fesenko
Feb 8, 1996·International Journal of Cancer. Journal International Du Cancer·A VillungerR Greil
Aug 12, 1999·Biotechnology Progress·D B Seifert, J A Phillips
Jan 27, 2000·Biophysical Chemistry·M F Chaplin
Apr 26, 2000·British Journal of Cancer·S G SenaratneK W Colston
Dec 6, 2000·Ultrasonics Sonochemistry·Y MizukoshiY Nagata
Mar 28, 2002·Biophysical Journal·Vladimir S MalininBarry R Lentz
Feb 8, 2003·Hybridoma and Hybridomics·Irena KirmanIlya Trakht
Oct 1, 2005·Journal of Nanoscience and Nanotechnology·Xiaobo ChenJames Becker
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Yaakov Levy, José N Onuchic
Sep 7, 2006·Nature Reviews. Molecular Cell Biology·Martin Chaplin