Enhancement of intracellular gamma-tocopherol levels in cytokine-stimulated C3H 10T1/2 fibroblasts: relation to NO synthesis, isoprostane formation, and tocopherol oxidation

BMC Chemical Biology
Yuichiro TanakaRobert V Cooney

Abstract

Stimulation of C3H 10T1/2 murine fibroblasts with interferon-gamma(IFN) and bacterial lipopolysaccharide (LPS) generates reactive oxygen and nitrogen species leading to DNA damage, lipid oxidation, and tocopherol oxidation. The tocopherols possess unique chemical and biological properties that suggest they have important roles related to intracellular defense against radical-mediated damage. Despite increased levels of reactive oxidants and decreased media tocopherol, cellular levels of gamma-tocopherol, but not alpha-tocopherol, were observed to increase significantly when cells were treated with IFN/LPS. Inhibition of nitric oxide (NO) synthesis by a specific inhibitor of inducible NO synthase (iNOS) increased both intracellular alpha-tocopherol and gamma-tocopherol concentrations, but did not significantly alter the reduction in media tocopherol levels caused by IFN/LPS treatment. Both exposure to exogenous NO and cellular synthesis of NO in cell culture increased media levels of 8-epi-prostaglandin F2alpha, a marker of oxidative lipid damage, whereas inhibition of endogenous NO synthesis reduced media 8-epi-prostaglandin F2alpha formation to control levels. Elevated intracellular levels of gamma-tocopherol in response to th...Continue Reading

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Citations

Jun 9, 2015·The British Journal of Nutrition·Yukiko MorimotoGertraud Maskarinec
Feb 15, 2012·Food & Function·Cherisse SenGertraud Maskarinec
Jan 21, 2020·Critical Reviews in Food Science and Nutrition·Liyou ZhengQingzhe Jin
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Apr 7, 2011·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Robert V CooneyWei Zheng

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Methods Mentioned

BETA
High Pressure Liquid Chromatography

Software Mentioned

GraphPad

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