Enhancement of LPS-induced microglial inflammation response via TLR4 under high glucose conditions

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Xiang ZhangYan-Ning Qian

Abstract

Microglia activation mediated by toll-like receptor 4 (TLR4) plays an important role in neuroinflammation and postoperative cognitive dysfunction (POCD). Diabetes mellitus (DM) has been recently suggested as an independent risk factor for POCD. In this study, we investigate the potential exacerbation of the inflammatory response in primary microglia due to high glucose conditions. Primary microglial cells were exposed to normal glucose (25 mmol/L) and high glucose (35 mmol/L) levels alone or with lipopolyscaccharide (LPS 0, 2, 5, 10 ng/mL). The pro-inflammatory response of the cells was assessed by measuring changes in cytokine levels and the evaluation of associated signaling pathways. Neither high glucose nor low LPS (≤5 ng/ml) alone had an effect on TNF-a and IL-6 levels, but the combination of low LPS and high glucose stimulated the inflammatory response. Analyses of the associated signaling pathways demonstrated that high glucose enhanced the LPS-induced microglial activation via the TLR4/JAK2/STAT3 pathway. This study demonstrates that high glucose, one of the key abnormalities characteristic of DM, can augment LPS-induced microglial activation and inflammatory cytokine levels through the TLR4/JAK2/STAT3 pathway, offering...Continue Reading

Citations

Feb 18, 2016·Neural Plasticity·Peng ZhouQing Wang
Jun 5, 2015·Frontiers in Cellular Neuroscience·Juhyun Song, Jong Eun Lee
Oct 1, 2016·Molecular and Cellular Endocrinology·Martin J T KalsbeekChun-Xia Yi
Jul 8, 2017·IUBMB Life·Nareshwaran GnanasegaranNoor Hayaty Abu Kasim
Dec 14, 2018·Frontiers in Cellular Neuroscience·Starlee LivelyLyanne C Schlichter
Apr 9, 2020·Frontiers in Immunology·Clotilde Lauro, Cristina Limatola
Jan 9, 2019·Frontiers in Cellular Neuroscience·Sara BachillerAntonio Boza-Serrano
Oct 15, 2019·Frontiers in Cellular Neuroscience·Clotilde LauroCristina Limatola
Nov 17, 2020·Frontiers in Cellular Neuroscience·Daniel C Shippy, Tyler K Ulland
Dec 17, 2020·International Journal of Molecular Sciences·Daniele LanaMaria Grazia Giovannini
Mar 5, 2021·Heliyon·Keitaro TachiMakoto Tanaka
Mar 16, 2021·Journal of Alzheimer's Disease : JAD·Ferdous TaslimaMakoto Michikawa
Feb 2, 2020·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Kelly E HenryJason S Lewis
May 17, 2021·Neurochemistry International·Aarti Sharma, Sidharth Mehan
Aug 24, 2021·Journal of Healthcare Engineering·Siriguleng SanaEnyou Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.