PMID: 45791Dec 1, 1978

Enhancement of macrophage adenylate cyclase by microtubule disrupting drugs

Immunopharmacology
A Grunspan-Swirsky, E Pick

Abstract

The accumulation of cyclic adenosine 3',5'-monophosphate (cAMP) in guinea-pig macrophages exposed to the adenylate cyclase (AC) stimulators prostaglandin E1 (PGE1) and isoproterenol (IP), was markedly enhanced by pretreatment of the cells with colchicine, vinblastine, and podophyllotoxin--agents which prevent microtubule assembly. The same agents did not augment basal cAMP levels. The facilitating effect of the drugs on the response to PGE1 and IP developed both in the absence and presence of a phosphodiesterase (PDE) inhibitor. The same drugs also enhanced the accumulation of cAMP induced by cholera toxin (CT) but the presence of a PDE inhibitor was required for such enhancement to become evident. Pretreatment of macrophages with cytochalasin B, an agent interfering with microfilament function, had no effect on the responsiveness of the cells to AC stimulators. The microtubule stabilizer, deuterium oxide (D2O) partially reversed the colchicine effect. Microtubule disrupting drugs did not block the release of cAMP from the cells into the surrounding medium. Macrophages incubated as monolayers or in suspension showed the same degree of increased responsiveness to stimulators after preexposure to colchicine. Preincubation with th...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·T HagaA G Gilman
Nov 4, 1976·The New England Journal of Medicine·L A BoxerR L Baehner
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S A RudolphS E Malawista
Jan 1, 1979·Experimental Cell Research·E Pick, I Wilner
Jan 1, 1976·Journal of Supramolecular Structure·G M Fuller, B R Brinkley
Dec 9, 1976·Nature·B Bhattacharyya, J Wolff
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·N Sahyoun, P Cuatrecasas
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J Orly, M Schramm
Oct 9, 1975·Nature·B B Walters, A I Matus
Jan 1, 1974·Annual Review of Biochemistry·P Cuatrecasas
May 1, 1970·The Journal of Infectious Diseases·R C DuhamelG F Grady

Citations

Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R SimantovL Sachs
Sep 1, 1981·The Journal of Cell Biology·R N MelmedR D Berlin
Nov 29, 1978·Biochemical and Biophysical Research Communications·A AdamE Lederer
Jan 1, 1979·Experimental Cell Research·E Pick, I Wilner
May 1, 1984·Archives of Biochemistry and Biophysics·L M Wahl, C C Winter
Jul 1, 1979·Cellular Immunology·A Grunspan-Swirsky, E Pick

Related Concepts

Calcimycin
Cyclic AMP, (R)-Isomer
Adenylate Cyclase
Caverject
Metazoa
Colchicine, (R)-Isomer
Cytochalasin B
Cavia porcellus
Novodrin
Microtubules

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