Enhancement of murine cardiac chronotropy by the molecular transfer of the human beta2 adrenergic receptor cDNA

The Journal of Clinical Investigation
Jay M EdelbergR D Rosenberg

Abstract

Cardiac pacemaking offers a unique opportunity for direct gene transfer into the heart. An experimental system was developed to assay the effects of transferring the human beta2 adrenergic receptor (beta2AR) under in vitro, ex vivo, and finally in vivo conditions. Constructs encoding either beta2AR or LacZ were used in chronotropy studies with isolated myocytes, and transplanted as well as endogenous murine hearts. Murine embryonic cardiac myocytes were transiently transfected with plasmid constructs. The total percentage of myocytes spontaneously contracting was greater in beta2AR transfected cells, as compared with control cells (67 vs. 42+/-5%). In addition, the percentage of myocytes with chronotropic rates > 60 beats per minute (bpm) was higher in the beta2AR population, as compared with control cells (37 vs. 15+/-5%). The average contractile rate was greater in the beta2AR transfected myocytes at baseline (71+/-14 vs. 50+/-10 bpm; P < 0.001) as well as with the addition of 10(-)3 M isoproterenol (98+/-26 vs. 75+/-18 bpm; P < 0.05). Based on these results, a murine neonatal cardiac transplantation model was used to study the ex vivo effects of targeted expression of beta2AR. The constructs were transfected into the right a...Continue Reading

References

Mar 23, 1990·Science·J A WolffP L Felgner
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R N KitsisL A Leinwand
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T A GustafsonE Morkin
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Kass-EislerL A Leinwand
Jan 15, 1997·The Journal of Clinical Investigation·M H DraznerR J Lefkowitz
Sep 1, 1963·The American Journal of Anatomy·R I FULMERA G LIEBELT

❮ Previous
Next ❯

Citations

Dec 17, 2010·Journal of Interventional Cardiac Electrophysiology : an International Journal of Arrhythmias and Pacing·Gerard J J Boink, Michael R Rosen
Dec 2, 2006·Medical & Biological Engineering & Computing·Traian M Anghel, Steven M Pogwizd
Jan 31, 2007·Medical & Biological Engineering & Computing·Yuichi TomitaKeiichi Fukuda
Aug 18, 2010·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jin MaXiaoqing Quan
Dec 29, 2000·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·S K Wattanapitayakul, J A Bauer
Sep 25, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Julien PironFlavien Charpentier
Jan 11, 2013·Nature Biotechnology·Edward G Lakatta, Victor A Maltsev
Sep 28, 2004·Nature Biotechnology·Izhak KehatLior Gepstein
Oct 17, 2001·Heart·J M EdelbergR D Rosenberg
Sep 6, 2006·Circulation·Douglas B Cowan, Francis X McGowan
Mar 2, 2011·Circulation·David S Park, Glenn I Fishman
Oct 28, 1998·The Journal of Clinical Investigation·S B LiggettR A Walsh
Oct 19, 2006·Medical & Biological Engineering & Computing·Gerard J J BoinkHanno L Tan
Feb 7, 2007·Medical & Biological Engineering & Computing·Jacques M T de Bakker, Antonio Zaza
Aug 21, 2013·Current Cardiology Reports·Evgeny PokushalovJonathan S Steinberg
Dec 2, 2005·Expert Opinion on Biological Therapy·Lior Gepstein
Oct 2, 2003·Expert Opinion on Biological Therapy·Jonathan Leor, Israel M Barbash
Aug 11, 2010·Journal of Molecular and Cellular Cardiology·Ian Greener, J Kevin Donahue
Apr 21, 2007·Heart, Lung & Circulation·Eddy Kizana
May 24, 2015·Trends in Cardiovascular Medicine·Gerard J J BoinkHanno L Tan
Apr 1, 2008·Annals of the New York Academy of Sciences·Andrea Barbuti, Dario DiFrancesco
Apr 1, 2008·Annals of the New York Academy of Sciences·Lior Gepstein
Sep 21, 2010·Transplant Immunology·Lijuan WangZhi Yao
Mar 30, 2010·Heart Failure Clinics·Roy BeinartDavid Milan
Apr 29, 2015·Nature Reviews. Cardiology·Rossana Bongianino, Silvia G Priori
Feb 10, 2009·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Lior Gepstein
Apr 27, 2005·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Michael R Rosen
Aug 17, 2011·Nature Reviews. Cardiology·Michael R RosenIra S Cohen
Aug 27, 2005·Nature Clinical Practice. Cardiovascular Medicine·Ira S CohenMichael R Rosen
Sep 17, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Michael R RosenIra S Cohen
Feb 20, 2010·Circulation Research·Dario DiFrancesco
Mar 6, 2010·Circulation Research·Hee Cheol Cho, Eduardo Marbán
Oct 20, 2011·Circulation. Arrhythmia and Electrophysiology·Philipp Sasse
Jan 21, 2012·Circulation·Crystal M Ripplinger, Donald M Bers
Feb 28, 2004·Circulation Research·Irina PotapovaIra S Cohen
Aug 23, 2006·Circulation·Annalisa BucchiMichael R Rosen
Mar 5, 2003·Circulation·Jihong QuMichael R Rosen
Sep 20, 2006·Pacing and Clinical Electrophysiology : PACE·Lior Yankelson, Lior Gepstein
Apr 26, 2007·Journal of Cardiovascular Electrophysiology·J Kevin Donahue

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.