Enhancement of mutanase production in Trichoderma harzianumby mutagenesis

Acta Biologica Hungarica
Adrian WiaterMałgorzata Pleszczyńska

Abstract

Conidia of Trichoderma harzianum F-340, an active producer of fungal mutanase, were mutagenized with physical and chemical mutagens used separately or in combination. After mutagenesis, the drop in conidia viability ranged from 0.004% to 71%. Among the applied mutagens, nitrosoguanidine gave the highest frequency of cultures with enhanced mutanase activity (98%). In total, 400 clones were isolated, and preliminarily evaluated for mutanase activity in flask microcultures. Eight most productive mutants were then quantified for mutanase production in shake flask cultures. The obtained results fully confirmed a great propensity of all the tested mutants to synthesize mutanase, the activity of which increased from 59 to 107% in relation to the parental T. harzianum culture. The best mutanase-overproducing mutant (T. harzianumn F-340-48), obtained with nitrosoguanidine, produced the enzyme activity of 1.36 U/ml (4.5 U/mg protein) after 4 days of incubation in shake flask culture. This productivity was almost twices higher than that achieved by the initial strain F-340, and, at present, is the best reported in the literature. The potential application of mutanase in dentistry is also discussed.

References

Mar 1, 1978·Scandinavian Journal of Dental Research·J KelstrupS Poulsen
Mar 1, 1972·Journal of Dental Research·B Guggenheim, R Haller
Feb 1, 1973·Analytical Biochemistry·G R Schacterle, R L Pollack
May 1, 1981·Canadian Journal of Microbiology·E R Stephen, A Nasim
Sep 15, 1993·FEMS Microbiology Letters·R G Quivey, P S Kriger
Dec 29, 2000·Carbohydrate Research·L Kandra, G Gyémánt
Jun 5, 2003·FEBS Letters·Lili KandraNarayanan Ramasubbu
Aug 5, 2003·Canadian Journal of Microbiology·N BalasubramanianD Lalithakumari
Feb 1, 1962·Journal of Bacteriology·M MANDELSE T REESE
Nov 26, 2003·Biochemical and Biophysical Research Communications·Gyöngyi GyémántLászló Somsák
Apr 6, 2004·FEMS Microbiology Letters·András SzekeresLászló Manczinger
Jun 15, 2004·Biochemical and Biophysical Research Communications·Lili KandraGyula Batta
Jan 1, 1945·The Biochemical Journal·P B Marshall, E W Rogers

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Citations

Jun 7, 2015·International Journal of Biological Macromolecules·Małgorzata PleszczyńskaJanusz Szczodrak

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