Enhancement of myogenic differentiation and inhibition of rhabdomyosarcoma progression by miR-28-3p and miR-193a-5p regulated by SNAIL.

Molecular Therapy. Nucleic Acids
Klaudia SkrzypekM Majka

Abstract

Rhabdomyosarcoma (RMS) is a soft tissue mesenchymal tumor that affects mostly children and adolescents. It originates from the impaired myogenic differentiation of stem cells or early progenitors. SNAIL, a transcription factor that regulates epithelial-to-mesenchymal transition in tumors of epithelial origin, is also a key regulator of RMS growth, progression, and myogenic differentiation. Here, we demonstrate that the SNAIL-dependent microRNAs (miRNAs) miR-28-3p and miR-193a-5p are crucial regulators of RMS growth, differentiation, and progression. miR-28-3p and miR-193a-5p diminished proliferation and arrested RMS cells in G0/G1 phase in vitro. They induced the myogenic differentiation of both RMS cells and human myoblasts by upregulating myogenic factors. Furthermore, miR-28-3p and miR-193a-5p inhibited migration in a scratch assay, adhesion to endothelial cells, chemotaxis, and invasion toward SDF-1 and HGF and regulated angiogenic capabilities of the cells. Overexpression of miR-28-3p and miR-193a-5p induced formation of fibrotic structures and abnormal blood vessels in RMS xenografts in immunodeficient mice in vivo. Simultaneous overexpression of both miRNAs diminished tumor growth after subcutaneous implantation and inhi...Continue Reading

References

Dec 26, 2001·Nucleic Acids Research·T HubbardM Clamp
Apr 18, 2007·Molecular and Cellular Biology·Huating WangDenis C Guttridge
Feb 6, 2010·Nature Medicine·Simone Hettmer, Amy J Wagers
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Jan 14, 2012·Gastroenterology·Maria I AlmeidaGeorge A Calin
Feb 4, 2012·Cold Spring Harbor Perspectives in Biology·C Florian BentzingerMichael A Rudnicki
Nov 7, 2013·European Journal of Human Genetics : EJHG·Homa TajsharghiAnders Oldfors
May 3, 2014·International Journal of Molecular Medicine·Danuta JarochaMarcin Majka
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Dec 18, 2015·Current Drug Targets·Barbara SzewczykMarcin Majka
Dec 26, 2015·Thrombosis Research·Xin ZhouYan Chen
Jun 17, 2016·Scientific Reports·Kuo-Wang TsaiHong-Tai Chang
Jun 25, 2016·In Vitro Cellular & Developmental Biology. Animal·Rui-Yao ZhuHe Huang
Jul 25, 2017·Biochemical and Biophysical Research Communications·Fan YiXiancan Wang
Nov 8, 2017·Scientific Reports·Falko M HeinemannJan U Becker
Dec 7, 2018·Journal of Cellular Biochemistry·Naghmeh ShirafkanBehzad Baradaran
Jan 9, 2019·Nature Reviews. Disease Primers·Stephen X SkapekDouglas S Hawkins
Feb 20, 2019·Journal of Cellular Physiology·Monireh KhordadmehrBehzad Baradaran
Dec 15, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Shusuke TodenAjay Goel

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TargetScanHuman
Integrative Genomics Viewer ( IGV )
ImageLab
cellSens Dimension
Attune NxT
GraphPad Prism
ImageJ
Ensembl

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