Enhancement of neurite-sprouting by suppression of HPC-1/syntaxin 1A activity in cultured vertebrate nerve cells

Brain Research
K YamaguchiKimio Akagawa

Abstract

HPC-1/syntaxin 1A is a C-terminal anchored neuronal membrane protein, of which all of the N-terminal regions are located on the intracellular side, and it interacts with presynaptic membrane proteins, synaptic vesicle proteins and soluble N-ethylmaleimide-sensitive fusion protein attachment proteins (SNAPs). HPC-1/syntaxin 1A has been proposed to act as a target SNAP receptor (t-SNARE) in the neuron and contributes to the vesicle docking/fusion process during the fast exocytosis at the presynaptic active zone. However, studies using an electron-microscope revealed that HPC-1/syntaxin 1A distributed not only at the presynaptic region but throughout the whole axonal membrane, and the functions of this axonal HPC-1/syntaxin 1A remain completely unknown. To investigate its physiological role, we attempted to inhibit the function of HPC-1/syntaxin 1A in cultured neural cells by following two methods. First, de novo synthesis of HPC-1/syntaxin 1A was inhibited by an application of antisense oligonucleotide in cultured adult rat dorsal root ganglion (DRG) neurons. Second, antibody against HPC-1/syntaxin 1A was applied intra-axonally in the cultured chick retinal ganglion neuron. Both treatments, which were expected to downregulate the...Continue Reading

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Citations

Jun 26, 2004·Cell Structure and Function·T J ItohH Hotani
Feb 10, 2016·Journal of Neural Transmission·Renata Basso CupertinoNina Roth Mota
Mar 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kazushi KimuraChizuka Ide
Aug 13, 1999·The American Journal of Physiology·B B BrimhallM E Sunday
Oct 29, 1998·Biochemical and Biophysical Research Communications·S ShimohamaT Taniguchi
Nov 18, 2000·Neuroscience Letters·M Fukuda, K Mikoshiba

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