Enhancement of periosteal bone formation by basic fibroblast-derived growth factor containing polycystic kidney disease and collagen-binding domains from Clostridium histolyticum collagenase

Journal of Tissue Engineering and Regenerative Medicine
Kentaro UchidaMasashi Takaso

Abstract

Recombinant basic fibroblast growth factor (bFGF) is a potent mitogen for mesenchymal cells that accelerates bone union and repair when applied locally at defect sites. However, because bFGF diffuses rapidly from bone defect sites, repeated dosing is required for sustained therapeutic effect. We previously fused the collagen-binding domain (CBD) and polycystic kidney disease (PKD) domain of Clostridium histolyticum class II collagenase (ColH) to bFGF and demonstrated that the fusion protein markedly enhances bone formation when loaded onto collagen materials used for grafting. However, systemic injection of a fusion protein consisting of parathyroid hormone (PTH) and a CBD was shown to accelerate bone formation in an osteoporosis model more rapidly than treatment with a PTH-PKD-CBD fusion protein. Here, we compared the biological properties of two collagen-binding forms of bFGF, bFGF-CBD and bFGF-PKD-CBD. Both fusion proteins promoted the in vitro proliferation of periosteal mesenchymal cells, indicating that they had biological activity similar to that of native bFGF. In vivo periosteal bone formation assays in rat femurs showed that both bFGF-CBD and bFGF-PKD-CBD induced periosteal bone formation at higher rates than collagen...Continue Reading

References

Mar 7, 1998·The Journal of Biological Chemistry·O MatsushitaA Okabe
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·N NishiF Wada
Jan 7, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T KatoK Nakamura
Jun 17, 2000·The American Journal of Cardiology·E F UngerA A Quyyumi
Jul 6, 2000·The Orthopedic Clinics of North America·S N KhanJ M Lane
Dec 31, 2000·The Journal of Biological Chemistry·O MatsushitaA Okabe
Feb 7, 2001·The Journal of Clinical Endocrinology and Metabolism·H KawaguchiM Tamura
Jan 6, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hiroshi KawaguchiKozo Nakamura
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hiroshi KawaguchiUNKNOWN TESK Group
Apr 23, 2011·Calcified Tissue International·Tulasi PonnapakkamR C Gensure
Aug 13, 2013·Drug Discovery Today·T PonnapakkamR C Gensure
Dec 12, 2013·The Journal of Investigative Dermatology. Symposium Proceedings·Ranjitha KatikaneniRobert Gensure
Jan 28, 2014·Biochimica Et Biophysica Acta·Youko FukataNozomu Nishi
Mar 12, 2015·Acta Crystallographica. Section D, Biological Crystallography·Ryan BauerJoshua Sakon

❮ Previous
Next ❯

Citations

Nov 9, 2016·Tissue Engineering. Part B, Reviews·Cyril AddiGregory De Crescenzo
Apr 25, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hisako FujimakiMasashi Takaso

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.