PMID: 8584016Oct 4, 1995Paper

Enhancement of phospholipid hydrolysis in vasopressin-stimulated BHK-21 and H9c2 cells

Molecular and Cellular Biochemistry
K TranP C Choy

Abstract

The hydrolysis of phospholipids in vasopressin-stimulated baby hamster kidney (BHK)-21 and H9c2 myoblastic cells was investigated. Phosphatidylcholine and phosphatidylethanolamine in these cells were pulse labelled with [3H]glycerol, [3H]myristate, [3H]choline or [3H]ethanolamine, and chased with the non-labelled precursor until linear turnover rates were obtained. When cells labelled with [3H]glycerol or [3H]myristate were stimulated by vasopressin, no significant decrease in the labelling of phosphatidylcholine was detected, but the labelling of phosphatidic acid was elevated. However, the labellings of phosphatidylethanolamine and its hydrolytic product were not affected by vasopressin stimulation. When the cells were pulse labelled with [3H]-choline, vasopressin stimulation caused a decrease in the labelled phosphatidylcholine with a corresponding increase in the labelled choline. The apparent discrepancy between the two types of labelling might be explained by the recycling of labelled phosphatidic acid back into phosphatidylcholine, thus masking the reduction in the labelled phospholipid during vasopressin stimulation. Alternatively, the labelled choline produced by vasopressin stimulation was released into the medium, th...Continue Reading

References

Dec 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·X ZhaP C Choy
Apr 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E A DennisY A Hannun
Jul 15, 1990·The Biochemical Journal·M M Billah, J C Anthes
Jan 1, 1989·Annual Review of Medicine·K J Catt, T Balla
Dec 30, 1988·Biochemical and Biophysical Research Communications·T W Martin, K C Michaelis
Jul 18, 1986·Science·Y Nishizuka
Jul 31, 1987·Biochemical and Biophysical Research Communications·Y T XuanW D Watkins
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M BestermanP Cuatrecasas

❮ Previous
Next ❯

Citations

Sep 27, 2001·Journal of Cellular Biochemistry·M A BrostromC O Brostrom
Nov 21, 2000·The International Journal of Biochemistry & Cell Biology·M A BrostromC O Brostrom
Mar 12, 2003·Biochemical Pharmacology·Francesca CeccarelliMaria R Mazzoni
Jan 15, 2005·Journal of Receptor and Signal Transduction Research·Laura GiustiMaria R Mazzoni
Jun 13, 1998·Progress in Lipid Research·P C ChoyE A Kroeger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.