Enhancement of procollagen biosynthesis by p180 through augmented ribosome association on the endoplasmic reticulum in response to stimulated secretion.

The Journal of Biological Chemistry
Tomonori UenoKiyoko Ogawa-Goto

Abstract

A coiled-coil microtubule-bundling protein, p180, was originally reported as a ribosome-binding protein on the rough endoplasmic reticulum (ER) and is highly expressed in secretory tissues. Recently, we reported a novel role for p180 in the trans-Golgi network (TGN) expansion following stimulated collagen secretion. Here, we show that p180 plays a key role in procollagen biosynthesis and secretion in diploid fibroblasts. Depletion of p180 caused marked reductions of secreted collagens without significant loss of the ER membrane or mRNA. Metabolic labeling experiments revealed that the procollagen biosynthetic activity was markedly affected following p180 depletion. Moreover, loss of p180 perturbs ascorbate-stimulated de novo biosynthesis mainly in the membrane fraction with a preferential secretion defect of large proteins. At the EM level, one of the most prominent morphological features of p180-depleted cells was insufficient ribosome association on the ER membranes. In contrast, the ER of control cells was studded with numerous ribosomes, which were further enhanced by ascorbate. Similarly biochemical analysis confirmed that levels of membrane-bound ribosomes were altered in a p180-dependent manner. Taken together, our data ...Continue Reading

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Citations

Jan 17, 2012·Molecular & Cellular Proteomics : MCP·Yuki TagaShunji Hattori
Dec 14, 2011·Nucleic Acids Research·Tomonori UenoKiyoko Ogawa-Goto
Jun 20, 2012·Clinical Proteomics·Deepthi TelikicherlaAkhilesh Pandey
Mar 20, 2014·Wiley Interdisciplinary Reviews. RNA·Xianying A Cui, Alexander F Palazzo
Mar 26, 2014·Current Opinion in Cell Biology·Naama Aviram, Maya Schuldiner
Apr 23, 2013·Biochimica Et Biophysica Acta·Yoshihiro Ishikawa, Hans Peter Bächinger
Nov 30, 2010·Cell·Yoko ShibataTom A Rapoport
Jun 25, 2016·Viruses·Inés Romero-Brey, Ralf Bartenschlager
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Jul 10, 2019·Bioscience Reports·Jing MaFanling Meng
Nov 15, 2015·The Journal of Biological Chemistry·Yuki TagaShunji Hattori
Apr 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tomonori UenoKiyoko Ogawa-Goto
Jul 27, 2014·The Journal of Biological Chemistry·Sujatha JagannathanChristopher V Nicchitta
Mar 26, 2021·British Journal of Cancer·Omprakash ShriwasRupesh Dash

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