Enhancement of protective efficacy through adenoviral vectored vaccine priming and protein boosting strategy encoding triosephosphate isomerase (SjTPI) against Schistosoma japonicum in mice

PloS One
Yang DaiYinchang Zhu

Abstract

Schistosomiasis japonica is a zoonotic parasitic disease; developing transmission blocking veterinary vaccines are urgently needed for the prevention and control of schistosomiasis in China. Heterologous prime-boost strategy, a novel vaccination approach, is more effective in enhancing vaccine efficacy against multiple pathogens. In the present study, we established a novel heterologous prime-boost vaccination strategy, the rAdV-SjTPI.opt intramuscular priming and rSjTPI subcutaneous boosting strategy, and evaluated its protective efficacy against Schistosoma japonicum in mice. Adenoviral vectored vaccine (rAdV-SjTPI.opt) and recombinant protein vaccine (rSjTPI) were prepared and used in different combinations as vaccines in a mouse model. The specific immune responses and protective efficacies were evaluated. Furthermore, the longevity of protective efficacy was also determined. Results showed that the rAdV-SjTPI.opt priming-rSjTPI boosting strategy elicited higher levels of specific IgG responses and broad-spectrum specific cellular immune responses. The protective efficacy could reach up to nearly 70% and 50% of protection could be observed at 10 weeks after the last immunization in mice. The rAdV-SjTPI.opt intramuscular pri...Continue Reading

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Citations

Dec 15, 2015·Expert Review of Vaccines·Adebayo J MolehinAfzal A Siddiqui
Mar 30, 2018·Frontiers in Cellular and Infection Microbiology·Christian StutzerChristine Maritz-Olivier
Oct 8, 2016·Parasites & Vectors·Biniam Mathewos TebejeDonald P McManus

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Methods Mentioned

BETA
ELISA
flow cytometry
transfection

Software Mentioned

SPSS
Elispot
JD801

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