Enhancement of radiosensitivity by roscovitine pretreatment in human non-small cell lung cancer A549 cells

Journal of Radiation Research
Feng ZhangQibing Mei

Abstract

Roscovitine has been reported to have anti-proliferative properties and is in process of undergoing clinical trials. In addition to its intrinsic anticancer properties, it has recently been suggested that roscovitine may also enhance the activity of traditional chemo- and radio- therapies in certain cancer cell lines. The purpose of this study was to define the activity of roscovitine in increasing radiosensitivity of human non-small cell lung cancer (NSCLC) cell line A549 cells in vitro. A549 cells were exposed to ionizing radiation (IR) of gamma-ray with or without roscovitine pretreatment. Clonogenic assay was performed and cell cycle and apoptosis were analyzed by flow cytometry. Expression of PARP, Ku70 and Ku80 proteins was detected by Western blot. The active form of caspase-3 positive cells were measured by flow cytometry. Our results showed that roscovitine caused dose-dependent apoptosis in A549 cells. Pretreatment with minimally toxic concentration of roscovitine significantly radiosensitized A549 cells by inhibiting colony formation. We then examined potential mechanisms that may contribute to the enhanced radiation response induced by roscovitine. Our results showed that the combination treatment significantly indu...Continue Reading

References

Sep 19, 2003·Journal of Radiation Research·Mitsumasa HashimotoHiroshi Utsumi
Jul 6, 2004·International Journal of Radiation Oncology, Biology, Physics·Timothy M Pawlik, Khandan Keyomarsi
Dec 14, 2004·Oncogene·Spencer J CollisAntony R Parker
Nov 22, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elvira CrescenziHugh J M Brady
Apr 4, 2006·Cell Cycle·Atan Gross

❮ Previous
Next ❯

Citations

Apr 30, 2010·Molecular Neurobiology·Alison Colquhoun
Dec 4, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cheng-Fang WangYong-Yan Wang
Jan 9, 2020·Open Biology·Samanta Sharma, Piotr Sicinski
May 28, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Angela B Y HuiFei-Fei Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis