Enhancement of radiosensitivity of wild-type p53 human glioma cells by adenovirus-mediated delivery of the p53 gene

Journal of Neurosurgery
F F LangP Tofilon

Abstract

The authors sought to determine whether combining p53 gene transfer with radiation therapy would enhance the therapeutic killing of p53 wild-type glioma cells. It has been shown in several reports that adenovirus-mediated delivery of the p53 gene into p53 mutant gliomas results in dramatic apoptosis, but has little effect on gliomas containing wild-type p53 alleles. Therefore, p53 gene therapy alone may not be a clinically effective treatment for gliomas because most gliomas are composed of both p53 mutant and wild-type cell populations. One potential approach to overcome this problem is to exploit the role p53 plays as an important determinant in the cellular response to ionizing radiation. In vitro experiments were performed using the glioma cell line U87MG, which contains wild-type p53. Comparisons were made to the glioma cell line U251MG, which contains a mutant p53 allele. Monolayer cultures were infected with an adenovirus containing wild-type p53 (Ad5CMV-p53), a control vector (dl312), or Dulbecco's modified Eagle's medium (DMEM). Two days later, cultures were irradiated and colony-forming efficiency was determined. Transfection with p53 had only a minor effect on the plating efficiency of nonirradiated U87MG cells, redu...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J KuerbitzM B Kastan
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E SetoT Shenk
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P ShawJ Costa
May 1, 1981·International Journal of Radiation Oncology, Biology, Physics·B Fertil, E P Malaise
Feb 24, 1995·Cell·E M MiyashitaD A Thorley-Lawson
Nov 1, 1993·International Journal of Radiation Biology·R E MeynL J Peters
Sep 17, 1996·International Journal of Cancer. Journal International Du Cancer·H KöckD C Maneval

❮ Previous
Next ❯

Citations

Oct 4, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Wei XiangHongyang Zhao
Oct 24, 2002·Current Treatment Options in Oncology·Stephen B Tatter
Dec 21, 2000·International Journal of Radiation Oncology, Biology, Physics·P J ColletierA Pollack
May 31, 2001·International Journal of Radiation Oncology, Biology, Physics·K OkamotoN Shitara
Feb 28, 2003·International Journal of Radiation Oncology, Biology, Physics·Waleed O ArafatDavid T Curiel
Apr 30, 2008·Neuro-oncology·Akira NakamizoFrederick F Lang
Dec 10, 1999·Journal of Neurosurgery·W C BroaddusH L Fillmore
Dec 1, 2001·Expert Opinion on Biological Therapy·J B Alavi, S L Eck
Nov 4, 2000·Expert Opinion on Investigational Drugs·N Gupta
Jul 22, 2006·Expert Opinion on Biological Therapy·Dmitry I Gabrilovich
Dec 9, 2008·International Journal of Radiation Biology·Linda S YasuiK R Saroja
Jan 9, 2003·Expert Opinion on Biological Therapy·C M F DirvenW P Vandertop
Jan 13, 2016·International Journal of Cancer. Journal International Du Cancer·Zhao HuangCanhua Huang
May 25, 2002·Cancer Treatment Reviews·J M KaminskiA Pollack
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·T W TraskR G Grossman
Jul 19, 2002·Cancer·Shoji ShiraishiYukitaka Ushio
May 2, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anthony W TolcherS Gail Eckhardt
Apr 6, 2018·Molecular Medicine Reports·Wenbo YangChangwu Dou
Jul 4, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Frederick F LangW K Alfred Yung
Aug 7, 2003·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Isamu KodaniHisao Ito
Jan 10, 2001·In Vitro Cellular & Developmental Biology. Animal·A NagpalS C Kaul
Nov 5, 1999·Neurosurgery·F F LangP J Tofilon
Jun 5, 2003·Technology in Cancer Research & Treatment·Tatyana M TimiryasovaIstvan Fodor
Nov 10, 2013·Cancers·Aneta KwiatkowskaMariano S Viapiano
Jun 21, 2020·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Yuko KinashiSentaro Takahashi
Sep 16, 1999·Annales Françaises D'anesthèsie Et De Rèanimation·V LaudenbachJ M Desmonts
Jul 27, 2021·Frontiers in Oncology·Xanthene MilesJulie Bolcaen
Aug 28, 2021·Brain Sciences·Alice Giotta Lucifero, Sabino Luzzi

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
Infection
Assay
electrophoresis

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.