PMID: 9177080Apr 1, 1997Paper

Enhancement of Rayleigh light scattering of acid chrome blue K by proteins and protein assay by the scattering technique

The Analyst
C Q MaS Tong

Abstract

Rayleigh light scattering of Acid Chrome Blue K (ACBK) is enhanced greatly by proteins. Based on this, a method for protein assay in aqueous solution was developed. This assay matches the sensitivity of the colorimetric dye-binding method with a linear range of 0.136-10.88 micrograms ml-1. The measurements can be made easily on a common fluorimeter. The reaction between ACBK and proteins is completed in 2 min and the scattered light signal is stable for at lest 3 h. Protein-to-protein variability is encountered in this method as in many other protein assays. There is little or no interference from amino acids, most metal ions and complexing agents (e.g., EDTA). Interferences from salts, urea and detergents can be minimized by dilution.

Citations

May 4, 2007·Bulletin of Environmental Contamination and Toxicology·Pinghua YangJinxiang Zeng
Apr 6, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Changxia SunShufang Liu
Jan 4, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Rutao LiuZengjun Lan
Dec 13, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xiufen LongHong Zhao
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Jun 13, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Changying GuoJinghe Yang
Dec 5, 2013·Luminescence : the Journal of Biological and Chemical Luminescence·Zhi-Ping CuiJing Tian
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Oct 6, 2005·Luminescence : the Journal of Biological and Chemical Luminescence·Xia WuTao Wu

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