Enhancement of star vector-based gene delivery to endothelial cells by addition of RGD-peptide

Bioconjugate Chemistry
Ayaka IshikawaYasuhide Nakayama

Abstract

This study aimed to investigate the feasibility of using a cationic nonviral gene carrier in endothelial cells for enhancing gene expression by the addition of an integrin-binding RGD peptide. A 4-branched cationic polymer of poly( N,N-dimethylaminopropylacrylamide) (star vector), developed as a gene carrier, could complex with the luciferase-encoding plasmid DNA under a charge ratio of 5 (vector/pDNA) to form polymer/DNA complexes (polyplexes). The addition of the RGD-containing peptide (GRGDNP) to the polyplex solution led to a decrease in the zeta-potential from ca. +30 to +20 mV along with the reduction in the particle size from ca. 300 to 200 nm. Additionally, a marked inhibition of polyplex aggregation was observed, indicating the coating of the polyplex surface with RGD peptides. A transfection study on endothelial cells showed that the luciferase activity increased with the amount of RGD peptides added to the polyplexes and exhibited minimal cellular cytotoxicity. The transfection activity further increased when cyclic RGD peptides (RGDFV) were used; the activity with RGD peptide addition was approximately 8-fold compared to that without RGD peptide addition. Gene delivery to endothelial cells was significantly enhanced...Continue Reading

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Citations

Feb 23, 2012·Accounts of Chemical Research·Yasuhide Nakayama
Aug 7, 2009·Journal of Biomechanical Engineering·Todd O PangburnEfrosini Kokkoli
Oct 6, 2011·Advanced Drug Delivery Reviews·Hyun-Ji ParkSeung-Woo Cho
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May 19, 2010·Organic & Biomolecular Chemistry·Jing-Xiao ChenRen-Xi Zhuo
Nov 15, 2008·Bioconjugate Chemistry·Yasushi NemotoYasuhide Nakayama
Mar 9, 2010·Bioconjugate Chemistry·Shubiao ZhangBing Wang

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