Enhancement of stress-induced synthesis of hsp27 and alpha B crystallin by modulators of the arachidonic acid cascade

Journal of Cellular Physiology
H ItoK Kato

Abstract

The regulation by intrinsic factors of responses to stress of two small stress proteins, hsp27 and alpha B crystallin, was examined in C6 rat glioma cells. Levels of hsp27 and alpha B crystallin were low in C6 glioma cells in confluent cultures. However, levels of the two proteins increased after exposure of cells to heat (42 degrees C for 30 min) or arsenite (50 microM for 1 h) stress. When cells were exposed to arsenite or hear in the presence of indomethacin (50 microM), an inhibitor of cyclooxygenase, or in the presence of nordihydroguaiaretic acid (NDGA; 50 microM), an inhibitor of lipoxygenase, induction of hsp27 and alpha B crystallin was markedly stimulated as detected by specific immunoassays, Western blot analysis, and Northern blot analysis. The presence of melittin (1 microM), an activator of phospholipase A2, during the stress period also stimulated the induction of the two proteins. The expression of hsp70 to each stress was also enhanced in the presence of indomethacin, NDGA, or melittin. The gel mobility shift assay revealed that these chemicals prolonged the arsenite-induced activation of heat shock element (HSE)-binding activity of heat shock transcriptional factor (HSF) in cells. Induction of hsp27 and alpha ...Continue Reading

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Citations

Dec 13, 2001·Neurochemistry International·Warren SuChung-Ren Jan
Apr 27, 1999·Progress in Retinal and Eye Research·B K Derham, J J Harding
Oct 26, 2000·Cell Stress & Chaperones·M Locke, J Atance
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Nov 3, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Minoru YoshidaOsamu Kozawa
Sep 25, 2004·Journal of Cellular Physiology·Hidenori ItoKanefusa Kato
May 3, 2005·Journal of Cellular Biochemistry·Hidenori ItoKanefusa Kato
Nov 10, 2006·The Journal of Biological Chemistry·Atsushi SanbeAkito Tanoue
Aug 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Kouseki HiradeToshihiko Uematsu
Dec 5, 1998·Biochemical and Biophysical Research Communications·M KitagawaY Ono

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