Enhancement of the antibody-dependent tumoricidal activity of human monocytes by human monocytic colony-stimulating factor

Japanese Journal of Cancer Research : Gann
S SuzuK Motoyoshi

Abstract

Human monocytic colony-stimulating factor (hM-CSF) stimulates mature cells as well as progenitor cells of human monocyte lineage. In the present study, we investigated the effect of completely purified native hM-CSF on the tumoricidal activity of human monocytes against Raji (Burkitt lymphoma-derived cell line) using a chromium release assay. Preincubation of human peripheral blood monocytes with hM-CSF for 48 h markedly enhanced their antibody-dependent cellular cytotoxicity (ADCC). This enhancing effect was dependent on the concentration of hM-CSF, and maximal enhancement was achieved at the concentration of 66 ng/ml. The number of receptor sites on monocytes against the Fc portion of immunoglobulin G increased 2- to 4-fold during 48 h incubation with hM-CSF. These results suggest that hM-CSF stimulates monocyte-ADCC by increasing the number of Fc receptor sites on human monocytes.

References

Feb 1, 1989·The Journal of Clinical Investigation·J C Unkeless
Jun 5, 1987·Science·S C Clark, R Kamen
Feb 1, 1982·The Journal of Clinical Investigation·E J WingK Stephenson

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Citations

May 1, 1991·Japanese Journal of Cancer Research : Gann·C YamashitaH Kiwada
Aug 7, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Takuma SakuraiKazuo Motoyoshi
Jan 1, 1994·Japanese Journal of Cancer Research : Gann·T SakuraiK Motoyoshi
Jun 16, 2000·Gastroenterology Nursing : the Official Journal of the Society of Gastroenterology Nurses and Associates·M L Ellett

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