Enhancement of the developmental potential of mouse oocytes matured in vitro by gonadotropins and ethylenediaminetetraacetic acid (EDTA)

Journal of in Vitro Fertilization and Embryo Transfer : IVF
M JinnoG D Hodgen

Abstract

We attempted to improve the developmental potential of mouse oocytes matured in vitro. First, the effect of gonadotropin supplementation of the oocyte maturation medium was tested. The addition of follicle-stimulating hormone (FSH) or luteinizing hormone (LH) alone significantly increased the rate of development of inseminated oocytes to two-cell embryos, resulting in a twofold increase in blastocyst development. There was no significant difference between FSH and LH supplementation. However, the beneficial effect of FSH or LH was abolished when both were added together. Next, we tested the effect of ethylenediaminetetraacetic acid (EDTA) supplementation of the embryo culture medium. The addition of 10 microM EDTA significantly enhanced the development of embryos derived from oocytes matured in vitro, both to two-cell embryos and to blastocysts. These data suggest that the inadequate development of embryos from oocytes matured in vitro results from a defect similar to that inherent in outbred mouse embryos showing the two-cell block in vitro.

References

Dec 1, 1987·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M JinnoR Iizuka
Sep 1, 1987·Biology of Reproduction·D LoutradisA A Kiessling
Jan 1, 1985·Annals of the New York Academy of Sciences·L L Veeck
Mar 4, 1967·Nature·D G Whittingham, J D Biggers
Apr 1, 1984·Developmental Biology·A C Schroeder, J J Eppig
Sep 30, 1982·Nature·A Muggleton-HarrisL Wilson

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Citations

Feb 1, 1990·Journal of in Vitro Fertilization and Embryo Transfer : IVF·N HolstH I Nielsen
Apr 1, 1997·Journal of Assisted Reproduction and Genetics·K L DurinziS E Lanzendorf
Dec 1, 1994·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D LoutradisA A Kiessling
Feb 1, 1992·Molecular Reproduction and Development·A I Younis, B G Brackett
Jan 1, 1993·Asian and Pacific Migration Journal : APMJ·A D Trlin

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