Enhancement of the insulin antagonistic effect of porcine somatotropin in swine using a monoclonal antibody

Domestic Animal Endocrinology
L A KraftB S Wang

Abstract

A monoclonal antibody (mAb), PS-7.6, to porcine somatotropin (pST) significantly enhanced the growth responses to pST injections in hypophysectomized (hypox) rats but could not be tested in pigs because of the large quantity of antibody required for a growth trial. Because pST inhibits the hypoglycemic effects of insulin, an insulin tolerance test procedure was established to measure pST activity in jugular-catheterized pigs. Doses of 0, 30, 100, and 300 micrograms/kg per day of pST were split and administered subcutaneously (sc) in equal portions twice daily for 2 d. After a 17-hr fast, plasma samples were obtained at 10-min intervals for 30 min before an intravenous injection of insulin (0.08 IU/kg) and then for an additional 50 min. Because pST increased fasting plasma glucose concentrations, preinsulin glucose values were used as a covariate to adjust the postinsulin concentrations. pST caused a dose-dependent increase in resistance to the insulin injection in these pigs. The areas under the curves (AUC), for plasma glucose were 22.1, 29.0, 39.0, and 47.2 mg/dl per min for the 0, 30, 100, and 300 micrograms/kg pST doses, respectively. Because different doses of pST could be detected, the PS-7.6 enhancement of pST treatment ...Continue Reading

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y ShechterP Cuatrecasas
Feb 1, 1989·The Journal of Endocrinology·J M PellR Aston
Oct 1, 1988·The Journal of Clinical Endocrinology and Metabolism·C F LimJ R Stockigt
May 1, 1970·The Journal of Clinical Endocrinology and Metabolism·T L GoodfriendJ S McGuire
Feb 1, 1968·Endocrinology·L J MachlinD M Kipnis

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