Abstract
Killer helper factor (KHF) was previously found to be produced by a human T cell hybridoma, 24A . CA2. We studied the therapeutic effects of interleukin-2 (IL-2) and KHF on the inhibition of pulmonary metastases of syngeneic Lewis lung carcinoma (3LL) in C57BL/6N mice. Multiple subcutaneous (sc) injections of IL-2 plus KHF had significantly more effect than injections of IL-2 alone in inhibiting spontaneous pulmonary metastases and prolonging survival of the mice. The effect of KHF with IL-2 on induction of lymphokine (IL-2)-activated killer (LAK) activity against P-29 cells was examined in the murine system. Spleen cells generated LAK activity after incubation for 4 days with more than 500 U/ml of IL-2. In contrast, KHF alone did not render spleen cells cytotoxic. The combination of these lymphokines at subthreshold concentrations, however, resulted in significant in vitro induction of LAK activity. The LAK activity of splenocytes incubated with IL-2 plus KHF was maximal after 4 days, and persisted for longer than that of cells treated with IL-2 alone. The LAK cells induced by KHF plus IL-2 were also cytotoxic to FBL and YAC-1 cells. Moreover, spleen cells of mice bearing lung metastases could be induced to the cytotoxic state...Continue Reading
References
Jan 1, 1985·Annual Review of Immunology·S K DurumJ J Oppenheim
Feb 1, 1988·Immunology Today·S A Rosenberg
Oct 1, 1986·The Journal of Experimental Medicine·J R OrtaldoR Overton
May 18, 1988·Journal of the National Cancer Institute·S SoneT Ogura
Jan 1, 1988·Cancer Immunology, Immunotherapy : CII·S YokotaS Kishimoto
Sep 15, 1988·International Journal of Cancer. Journal International Du Cancer·S SoneT Ogura
Apr 9, 1987·The New England Journal of Medicine·S A RosenbergJ T Rubin
Apr 9, 1987·The New England Journal of Medicine·W H WestR K Oldham
Sep 15, 1987·International Journal of Cancer. Journal International Du Cancer·M J BrundaV Sulich
Jan 1, 1985·Cancer Immunology, Immunotherapy : CII·M YagiE Tsubura
May 1, 1985·The Journal of Experimental Medicine·S A RosenbergS L Schwarz
Dec 5, 1985·The New England Journal of Medicine·S A RosenbergJ T Vetto
May 28, 1981·Nature·C S HenneyS Gillis
Jun 1, 1982·The Journal of Experimental Medicine·E A GrimmS A Rosenberg
Sep 28, 1984·Science·J J MuléS A Rosenberg
Mar 1, 1983·The Journal of Experimental Medicine·E A GrimmS A Rosenberg
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T R BeardsleyE F Hays
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Gillis, S B Mizel
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M OkadaT Kishimoto
Apr 22, 1982·Nature·D H Raulet, M J Bevan
Jun 1, 1982·The Journal of Experimental Medicine·H WagnerK Pfizenmaier