PMID: 9193791May 1, 1997Paper

Enhancement of thymic lymphocyte proliferation by the culture supernatant of thymus epithelial cells stimulated by prolactin

Cellular and Molecular Biology
B LinY Tsuji

Abstract

Culture supernatant of thymus epithelial cells (TECs) stimulated by prolactin (PRL) enhanced markedly DNA synthetic activity of thymic lymphocytes (TLs) as compared with the hormone-non-stimulated TECs. The supernatant, which was treated with anti-insulin-like growth factor-I (IGF-I) monoclonal antibody (MAb) (binding specifically to C region of IGF-I) has still the capacity of enhancing remarkably TL-proliferation. However, further treatment by ultrafiltration of the MAb-treated supernatant, removing the immune complex (IGF-I and anti-IGF-I MAb) from the supernatant, suppressed significantly the enhanced proliferation of TLs. It is assumed that PRL, like growth hormone (GH), promotes the release of IGF-I from TECs which induces a marked TL-proliferation. Moreover, it seems that the active site for inducing the proliferation is a region different from C, possibly the A or the B regions. TLs, present at different maturation steps, were separated into three subsets by discontinuous density gradient centrifugation and then treated with the supernatant of PRL-stimulated TECs. The least dense subset containing precursor T-cells and the most immature TLs showed the highest proliferative response to the supernatant in the comparison w...Continue Reading

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