PMID: 2503524Aug 1, 1989Paper

Enhancement of vinculin synthesis by migrating stratified squamous epithelium

The Journal of Cell Biology
James D ZieskeI K Gipson

Abstract

A 110-115-kD protein is present at levels 27-fold higher in migratory epithelium in the rat cornea than in stationary epithelium. This protein represents 2.7% of the total protein in migratory epithelium 6-h postabrasion wound and 0.1% of the total protein in stationary epithelium. Our findings demonstrate that this 110-115-kD protein is vinculin. In Western blots comparing proteins from migratory and control epithelium, antibody against vinculin cross-reacted with the 110-115-kD protein. Using immunoslot blots, vinculin was determined to be present at maximal levels 6 h postabrasion wound, at levels 22- and 8-fold higher than control at 18 and 48 h, respectively, returning to control levels 72 h postwounding. Vinculin was also localized by indirect immunohistochemistry in migrating corneal epithelium. 3-mm scrape wounds were allowed to heal in vivo for 20 h. In flat mounts of these whole wounded corneas, vinculin was localized as punctate spots in the leading edge of migrating epithelium. In cryostat sections, vinculin was localized as punctate spots along the basal cell membranes of the migrating sheet adjacent to the basement membrane and in patches between cells as well as diffusely throughout the cell. Only very diffuse lo...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1986·The Journal of Cell Biology·J A Wilkins, S Lin
Feb 1, 1987·Experimental Cell Research·G M AsyeeL Muszbek
Jan 1, 1987·Journal of Cell Science. Supplement·B GeigerR Bendori
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Aug 8, 1983·FEBS Letters·V E Koteliansky, G N Gneushev
Apr 19, 1984·Nature·K Burridge, P Mangeat
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B GeigerS J Singer
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B M Jockusch, G Isenberg
Jul 1, 1982·Developmental Biology·I K Gipson, T C Kiorpes
Nov 1, 1960·Stain Technology·K C RICHARDSONE H FINKE

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Citations

Jan 3, 2001·Journal of Cellular Biochemistry·Q H SongV Trinkaus-Randall
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M A SteppI K Gipson
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·J L CollE D Adamson
Jan 25, 2000·The Journal of Biological Chemistry·S SaikaW W Kao
Oct 1, 1992·The Journal of Cell Biology·J L Rodríguez FernándezA Ben-Ze'ev
Dec 1, 1992·The Journal of Cell Biology·M L ChiuE J O'Keefe
Dec 14, 2011·PloS One·Courtney MankusVickery Trinkaus-Randall
Jun 25, 1998·Cell Adhesion and Communication·A TozerenS W Byers
Jan 1, 1991·Current Eye Research·L S Grushkin-Lerner, V Trinkaus-Randall
Jan 1, 1992·Cell Motility and the Cytoskeleton·J L Rodríguez FernándezA Ben-Ze'ev
Sep 12, 2009·Acta histochemica·Shamim MushtaqNikhat Ahmed
Apr 1, 2008·Biochemical and Biophysical Research Communications·Ji-Ae KoTeruo Nishida
Jul 18, 1997·Biochemical and Biophysical Research Communications·P A Coulombe
Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M A Stepp, L Zhu
Mar 13, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Obianamma E OnochieVickery Trinkaus-Randall
Feb 1, 1992·Annals of Medicine·T TervoK Tervo
Jan 1, 1992·Acta Ophthalmologica. Supplement·I K Gipson
Jul 1, 2010·Molecular and Cellular Biochemistry·Amy LaiAziz Ghahary
May 9, 2002·Cornea·Shizuya SaikaWinston Whei-Yang Kao
Jul 11, 2001·Experimental Biology and Medicine·L LuW W Kao
Dec 1, 1993·The Journal of Investigative Dermatology·H W KaiserH W Kreysel
Jun 1, 1993·The Journal of Investigative Dermatology·M D Kubler, F M Watt

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