PMID: 12753592May 20, 2003Paper

Enhancement of virus-induced gene silencing through viral-based production of inverted-repeats

The Plant Journal : for Cell and Molecular Biology
Christophe LacommeIngo Hein

Abstract

Plant virus-based vectors carrying sequences homologous to endogenous genes trigger silencing through a homology-dependent RNA degradation mechanism. This phenomenon, called virus-induced gene silencing (VIGS), has potential as a powerful reverse-genetics tool in functional genomic programmes through transient, loss-of-function screens. Here, we describe a method to enhance the robustness of the VIGS phenotype by increasing the level of dsRNA molecule production, a critical step in the VIGS response. Incorporation of 40-60 base direct inverted-repeats into a plant viral vector generates RNA molecules that form dsRNA hairpins. A tobacco mosaic virus (TMV)-based vector carrying such inverted-repeats, homologous to a green fluorescent protein (gfp) transgene or an endogenous phytoene desaturase (pds) gene, generated a stronger and more pervasive VIGS phenotype than constructs carrying corresponding cDNA fragments in sense or antisense orientation. Real-time RT-PCR indicated that there was up to a threefold reduction in target mRNA accumulation in the tissues where VIGS was triggered by constructs carrying inverted-repeats compared to those where it was triggered by sense or antisense constructs. Moreover, an enhanced VIGS pds phen...Continue Reading

References

Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M H KumagaiL K Grill
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J HaseloffS Hodge
Aug 18, 1997·Virology·P D Nagy, A E Simon
Jun 23, 1998·The Plant Cell·M T RuizD C Baulcombe
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·P M WaterhouseM B Wang
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K W Buck
May 14, 1999·Current Opinion in Plant Biology·D C Baulcombe
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·C Lacomme, S Santa Cruz
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·O VoinnetD C Baulcombe
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C F Chuang, E M Meyerowitz
Sep 22, 2000·Plant Molecular Biology·M W MuskensJ M Kooter
Nov 23, 2000·Current Opinion in Genetics & Development·C Cogoni, G Macino
Jan 3, 2001·The Plant Journal : for Cell and Molecular Biology·P SchweizerR Dudler
Jan 12, 2001·Plant Physiology·S HauptK J Oparka
Feb 13, 2001·The Plant Journal : for Cell and Molecular Biology·F RatcliffD C Baulcombe
Feb 24, 2001·Nature·D Baulcombe
Jun 19, 2001·Molecular Genetics and Genomics : MGG·A F IbrahimC G Simpson
Aug 4, 2001·Trends in Genetics : TIG·O Voinnet
Sep 29, 2001·The Plant Journal : for Cell and Molecular Biology·S V WesleyP M Waterhouse
Nov 17, 2001·Journal of Virology·F Tenllado, J R Díaz-Ruíz
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Steve HolzbergGregory P Pogue
Jun 6, 2002·The Plant Journal : for Cell and Molecular Biology·Rachel L TothSean Chapman
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·M. Fagard, H. Vaucheret
Sep 1, 1998·The Plant Journal : for Cell and Molecular Biology·Andrew J HamiltonDon Grierson

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Citations

May 22, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Lijuan WangXiaoquan Qi
Oct 17, 2007·Plant Cell Reports·Mandar R GodgePrakash P Kumar
Sep 28, 2010·Planta·Arunima PurkayasthaIndranil Dasgupta
Sep 22, 2011·Functional & Integrative Genomics·Harvinder S BennypaulKulvinder S Gill
Jun 9, 2005·Current Opinion in Plant Biology·Patrice Dunoyer, Olivier Voinnet
Jun 23, 2004·Journal of Experimental Botany·Claire GachonBénédicte Charrier
Jul 25, 2009·Journal of Experimental Botany·G DubreuilM N Rosso
Nov 1, 2006·Molecular Plant-microbe Interactions : MPMI·Xin Shun DingRichard S Nelson
Nov 6, 2007·Molecular Plant-microbe Interactions : MPMI·Marianne Bruun-RasmussenMerete Albrechtsen
Jul 16, 2008·Molecular Plant-microbe Interactions : MPMI·G D ConstantinO S Lund
Apr 16, 2004·Plant Physiology·Odile Faivre-RampantChristophe Lacomme
Dec 14, 2004·Plant Physiology·Tracy ValentineChristophe Lacomme
Nov 6, 2007·Plant Physiology·Axel HimmelbachJochen Kumlehn
Jan 8, 2009·Plant Physiology·Steven R Scofield, Richard S Nelson
Feb 3, 2007·Journal of Virology·Vitantonio PantaleoJózsef Burgyán
Apr 30, 2009·Annual Review of Phytopathology·Andrew O JacksonMi Yeon Lee
Sep 21, 2004·Annual Review of Plant Biology·Dominique Robertson
Jun 24, 2009·International Journal of Plant Genomics·Turgay Unver, Hikmet Budak
Apr 15, 2011·Uirusu·Noriko Yamagishi, Nobuyuki Yoshikawa
Oct 31, 2013·Plant Cell Reports·Xionghui ZhongMingfang Yi
Mar 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tiziana MasciaDonato Gallitelli
Sep 6, 2005·FEBS Letters·John M WatsonPeter M Waterhouse
Sep 23, 2011·Trends in Plant Science·Muthappa Senthil-Kumar, Kirankumar S Mysore
Mar 15, 2012·Science China. Life Sciences·Changjun HuangXueping Zhou
Nov 12, 2009·Journal of Virological Methods·Cristiano LacorteMarcel Prins
Sep 29, 2009·Plant Physiology and Biochemistry : PPB·Arunima Purkayastha, Indranil Dasgupta
Apr 7, 2009·Biochimica Et Biophysica Acta·Christian Toft MadsenMerete Albrechtsen

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