Enhancement of whole cell synaptic currents by low osmolarity and by low [NaCl] in rat hippocampal slices

Journal of Neurophysiology
R HuangG G Somjen

Abstract

We recorded whole cell currents of patch-clamped neurons in stratum pyramidale of CA1 region of rat hippocampal tissue slices. Synaptic currents were evoked by orthodromic stimulation while holding potential of the neuron was varied from hyperpolarized to depolarized levels. Extracellular osmolarity (pi(o)) was lowered by superfusion with artificial cerebrospinal fluid in which NaCl concentration ([NaCl]) was reduced. The effect of low extracellular NaCl was tested in additional trials in which NaCl was substituted by isosmolar fructose. Both lowering of pi(o) and isosmotic lowering of extracellular [NaCl] ([NaCl]o) caused reversible increase of excitatory postsynaptic currents. The effect of lowering pi(o) was concentration dependent, and it was significantly stronger than the effect of equivalent isosmotic lowering of [NaCl]o. Inhibitory postsynaptic currents also increased in many but not in all cases. Lowering of pi(o) caused a prolongation of the time constant of relaxation of the capacitive charging current induced by small hyperpolarizing voltage steps. A virtual input capacitance, calculated by dividing this time constant by the input resistance, increased during hypotonic exposure. Isosmotic lowering of [NaCl]o had no ...Continue Reading

References

Jan 1, 1991·Journal of the Neurological Sciences·R D Andrew
Jan 1, 1990·Neuroscience·A S Rosen, R D Andrew
Jun 1, 1990·The Journal of General Physiology·C R FrenchP W Gage
Dec 1, 1989·Journal of Neuroscience Methods·M G BlantonA R Kriegstein
Sep 25, 1989·Brain Research·R D AndrewA S Rosen
Mar 1, 1968·The Journal of Physiology·B Katz, R Miledi
Dec 31, 1993·Brain Research·G G SomjenW J Wadman
Mar 1, 1994·Progress in Neurobiology·K Kaila
Jan 17, 1994·Neuroscience Letters·P J Zhu, K Krnjević
Jul 1, 1964·Journal of Neurophysiology·P ANDERSENY LOYNING
Jul 1, 1964·Journal of Neurophysiology·P ANDERSENY LOYNING

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Citations

Jun 14, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·George G Somjen
May 13, 2004·Journal of Neuroscience Research·Ryuichi NakajimaYoshihisa Kudo
Apr 27, 2011·Journal of Neurochemistry·María C Hyzinski-GarcíaAlexander A Mongin
Apr 18, 1998·Journal of Neurophysiology·S C Baraban, P A Schwartzkroin
Jul 27, 2017·Glia·Brian Roland Larsen, Nanna MacAulay
Mar 11, 2004·The European Journal of Neuroscience·Karina TuzHerminia Pasantes-Morales
Sep 6, 2000·Journal of Neuroscience Research·R FrancoH Pasantes-Morales
Jan 27, 2005·Neurochemistry International·Tatyana V WaseemSergei V Fedorovich

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