Enhancer action in trans is permitted throughout the Drosophila genome

Proceedings of the National Academy of Sciences of the United States of America
Ji-Long ChenPamela K Geyer

Abstract

Interactions between paired homologous genes can lead to changes in gene expression. Such trans-regulatory effects exemplify transvection and are displayed by many genes in Drosophila, in which homologous chromosomes are paired somatically. Transvection involving the yellow cuticle pigmentation gene can occur by at least two mechanisms, one involving the trans-action of enhancers on a paired promoter and a second involving pairing-mediated bypass of a chromatin insulator. A system was developed to evaluate whether the action of the yellow enhancers in trans could be reconstituted outside of the natural near telomeric location of the yellow gene. To this end, transgenic flies were generated that carried a yellow gene modified by the inclusion of strategically placed recognition sites for the Cre and FLP recombinases. Independent action of the recombinases produced a pair of derivative alleles, one enhancerless and the other promoterless, at each transgene location. Transvection between the derivatives was assessed by the degree of interallelic complementation. Complementation was observed at all eight sites tested. These studies demonstrate that yellow transvection can occur at multiple genomic locations and indicate that the Dr...Continue Reading

References

Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P K GeyerM M Green
Aug 1, 1984·Cell·R E Karess, G M Rubin
Oct 22, 1982·Science·G M Rubin, A C Spradling
Sep 24, 1983·Nucleic Acids Research·G M Rubin, A C Spradling
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W M Gelbart
Dec 1, 1994·Genetics·W M LeisersonS Benzer
May 3, 1996·Science·J M LaSalle, M Lalande
Jul 12, 1996·Cell·R Aramayo, R L Metzenberg
Nov 1, 1996·Genetics·A MochizukiY Iwasa
Oct 23, 1997·Genes & Development·H L AsheN J Proudfoot
May 20, 1998·Cell·S Henikoff, L Comai
Aug 26, 1998·Genes & Development·B Rockmill, G S Roeder
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R MorrisC T Wu
May 14, 1999·Current Opinion in Genetics & Development·C T Wu, J R Morris
May 14, 1999·Current Opinion in Genetics & Development·M Cockell, S M Gasser
May 26, 1999·Results and Problems in Cell Differentiation·W F Marshall, J W Sedat
Aug 24, 1999·Biochimica Et Biophysica Acta·V Pirrotta
Oct 29, 1999·Genes & Development·Y Marahrens
Mar 25, 2000·Science·M D AdamsJ C Venter
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·F L SunS C Elgin
Apr 5, 2002·Advances in Genetics·James A Kennison, Jeffrey W Southworth

❮ Previous
Next ❯

Citations

Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·M C AngueraJ T Lee
Nov 1, 2005·Plant Physiology·Wei YangUeli Grossniklaus
Aug 2, 2006·Molecular and Cellular Biology·Timothy J ParnellPamela K Geyer
Sep 16, 2004·Molecular and Cellular Biology·Toshio IshikawaJ Larry Jameson
Oct 18, 2005·Molecular and Cellular Biology·Elena KravchenkoMikhail Savitsky
Aug 16, 2008·PLoS Genetics·Alexey A SoshnevPamela K Geyer
Jun 10, 2005·Genetics·Alistair B CoulthardArthur J Hilliker
Jul 22, 2011·Genetics·Alexey A SoshnevPamela K Geyer
Jun 1, 2012·Genetics·David J Mellert, James W Truman
Jun 1, 2012·Genetics·Jack R BatemanMelissa N Locke
May 9, 2012·Genetics Research International·Manasi S Apte, Victoria H Meller
Nov 12, 2013·FEBS Letters·Olga Kyrchanova, Pavel Georgiev
Dec 7, 2010·Developmental Biology·Maximilian Haeussler, Jean-Stéphane Joly
Nov 14, 2002·Annual Review of Genetics·Ian W Duncan
Jan 1, 2004·Toxicology Mechanisms and Methods·Supratim Choudhuri
Mar 18, 2016·Genetics·Amanda J BlickJack R Bateman
Apr 6, 2019·Genetics·Pawel PiwkoChristos Delidakis
Oct 16, 2019·Genetics·Thomas D KingJack R Bateman
Feb 26, 2016·PLoS Genetics·Miki FujiokaJames B Jaynes
Oct 5, 2019·Nature Communications·Jumana AlHaj AbedC-Ting Wu
Sep 13, 2014·G3 : Genes - Genomes - Genetics·Xinyang BingThomas J S Merritt
Oct 13, 2018·Scientific Reports·Mo LiHaini N Cai
Jan 6, 2006·Doklady. Biochemistry and Biophysics·S A Rodin, P G Georgiev
Apr 6, 2005·Human Molecular Genetics·Adam G West, Peter Fraser
Aug 5, 2016·G3 : Genes - Genomes - Genetics·Geoffrey P NobleSurachai Supattapone
Dec 1, 2019·Genetics·Thomas D KingJack R Bateman
Jun 3, 2021·Genes·Samantha C PetersonStacey L Hanlon
Apr 19, 2003·Genetics·Mikhail SavitskyPavel Georgiev

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.