Enhancer analysis by chicken embryo electroporation with aid of genome comparison

Development, Growth & Differentiation
Masanori Uchikawa

Abstract

The identification of the enhancers associated with each developmentally regulated gene is a first step to clarify the regulatory mechanisms underlying embryogenesis. The electroporation technique using chicken embryo is a powerful tool to identify such enhancers. The technique enables us to survey a large genomic region and to analyze the enhancers in great detail. Comparison of the genomic sequences of the chicken and other vertebrate species identifies conserved non-coding sequence blocks to which the functionally identified enhancers often correspond. In this review, I describe in detail the methods to analyze the enhancers using the chicken embryo electroporation and genome comparison.

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Citations

Feb 19, 2009·Development, Growth & Differentiation·Eiji Matsunaga, Kazuo Okanoya
Apr 23, 2009·Development, Growth & Differentiation·Hajime Ogino, Haruki Ochi
May 29, 2010·Development, Growth & Differentiation·Yuka SaigouMasanori Uchikawa
Oct 5, 2010·Development, Growth & Differentiation·Jun TanakaHarukazu Nakamura
Jun 29, 2012·Neural Development·M Natalia Vergara, M Valeria Canto-Soler
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Nov 20, 2012·Development, Growth & Differentiation·Harukazu Nakamura, Junichi Funahashi
Nov 3, 2009·Genesis : the Journal of Genetics and Development·J Andrew Gillis, Neil H Shubin
Nov 29, 2014·Development, Growth & Differentiation·Ryuji OkamotoHisato Kondoh
Jun 5, 2012·Developmental Biology·Xiaohong SongKunimasa Ohta
Jan 31, 2020·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideaki IidaHisato Kondoh
Aug 3, 2012·Cold Spring Harbor Protocols·Charmaine Y BrownSeema Agarwala

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