Enhancer and silencerlike sites within the transcribed portion of a Ty2 transposable element of Saccharomyces cerevisiae.

Molecular and Cellular Biology
P J FarabaughJ Clare

Abstract

The Ty2-917 element is a member of the Ty2 class of retroviruslike transposable elements of Saccharomyces cerevisiae. We showed that regions downstream of the Ty2-917 transcription start site modulate its transcription. One region was located downstream of the transcription initiation site (position 240) and within the first 559 base pairs of the element. This region had a dramatic effect, causing an approximately 1,000-fold increase in steady-state levels of RNA. The region stimulated transcription when placed in either orientation upstream of a heterologous gene, HIS4, lacking its own upstream activation sequence (UAS). We termed this positively acting region an enhancer, by analogy to sites described in higher cells, to distinguish it from yeast UASs which do not function when placed within the transcribed portion of the gene. Though, like some higher eucaryotic enhancers, the Ty2-917 enhancer is located within the transcribed region, it is unlike them in that it occurs within a coding region rather than in an intron. The Ty2-917 enhancer and the Ty2-917 UAS had a synergistic effect on transcription, together stimulating transcription 15-fold over the predicted additive effect. We also identified a site which decreases RNA a...Continue Reading

References

May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J Clare, P Farabaugh
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·X B LiaoP J Farabaugh
Jun 24, 1988·Nucleic Acids Research·A M FultonA J Kingsman
Mar 1, 1985·Cell·J D BoekeG R Fink
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B ErredeW S Yarnell
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G S RoederR E Pearlman
Sep 25, 1985·Nucleic Acids Research·J R WarmingtonS G Oliver
Oct 20, 1988·Nature·M Ptashne
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Guarente, E Hoar
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K Struhl
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R T ElderR W Davis
Sep 19, 1980·Science·G S RoederG R Fink
Jan 1, 1981·Molecular & General Genetics : MGG·M Ciriacy, V M Williamson
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J L Teem, M Rosbash
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura

❮ Previous
Next ❯

Citations

Aug 19, 2015·Nucleic Acids Research·Hasan TükenmezAnders S Byström
Mar 22, 2001·Molecular and Cellular Biology·Q Li, S A Johnston
Dec 1, 1990·Molecular and Cellular Biology·G D Swergold

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.