Enhancer blocking and transvection at the Drosophila apterous locus.

Genetics
Daryl GohlP Schedl

Abstract

Intra- and interchromosomal interactions have been implicated in a number of genetic phenomena in diverse organisms, suggesting that the higher-order structural organization of chromosomes in the nucleus can have a profound impact on gene regulation. In Drosophila, homologous chromosomes remain paired in somatic tissues, allowing for trans interactions between genes and regulatory elements on the two homologs. One consequence of homolog pairing is the phenomenon of transvection, in which regulatory elements on one homolog can affect the expression of a gene in trans. We report a new instance of transvection at the Drosophila apterous (ap) locus. Two different insertions of boundary elements in the ap regulatory region were identified. The boundaries are inserted between the ap wing enhancer and the ap promoter and have highly penetrant wing defects typical of mutants in ap. When crossed to an ap promoter deletion, both boundary inserts exhibit the interallelic complementation characteristic of transvection. To confirm that transvection occurs at ap, we generated a deletion of the ap wing enhancer by FRT-mediated recombination. When the wing-enhancer deletion is crossed to the ap promoter deletion, strong transvection is observe...Continue Reading

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Citations

Apr 8, 2011·Nature Methods·Daryl M GohlThomas R Clandinin
Dec 14, 2011·Nature Structural & Molecular Biology·Emmanuel CaussinusMarkus Affolter
Nov 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Swagatam MukhopadhyayAnirvan M Sengupta
Nov 26, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Michèle Amouyal
Jun 1, 2012·Genetics·David J Mellert, James W Truman
Jun 1, 2012·Genetics·Jack R BatemanMelissa N Locke
Jan 31, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Darya ChetverinaPaul Schedl
Feb 18, 2017·ELife·Yvette E FisherThomas R Clandinin
Mar 18, 2016·Genetics·Amanda J BlickJack R Bateman
Apr 4, 2015·G3 : Genes - Genomes - Genetics·Dimitri BieliMartin Müller

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