Enhancing Action of Positive Allosteric Modulators through the Design of Dimeric Compounds

Journal of Medicinal Chemistry
Thomas DrapierBernard Pirotte

Abstract

The present study describes the identification of highly potent dimeric 1,2,4-benzothiadiazine 1,1-dioxide (BTD)-type positive allosteric modulators of the AMPA receptors (AMPApams) obtained by linking two monomeric BTD scaffolds through their respective 6-positions. Using previous X-ray data from monomeric BTDs cocrystallized with the GluA2 ligand-binding domain (LBD), a molecular modeling approach was performed to predict the preferred dimeric combinations. Two 6,6-ethylene-linked dimeric BTD compounds (16 and 22) were prepared and evaluated as AMPApams on HEK293 cells expressing GluA2o( Q) (calcium flux experiment). These compounds were found to be about 10,000 times more potent than their respective monomers, the most active dimeric compound being the bis-4-cyclopropyl-substituted compound 22 [6,6'-(ethane-1,2-diyl)bis(4-cyclopropyl-3,4-dihydro-2 H-1,2,4-benzothiadiazine 1,1-dioxide], with an EC50 value of 1.4 nM. As a proof of concept, the bis-4-methyl-substituted dimeric compound 16 (EC50 = 13 nM) was successfully cocrystallized with the GluA2o-LBD and was found to occupy the two BTD binding sites at the LBD dimer interface.

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Citations

Dec 6, 2019·MedChemComm·Anna A NazarovaVladimir A Palyulin
May 4, 2021·The Journal of Physiology·Karla FrydenvangJette Sandholm Kastrup
Jun 19, 2021·Biomedit︠s︡inskai︠a︡ khimii︠a︡·E V RadchenkoV A Palyulin
Jul 9, 2021·Organic & Biomolecular Chemistry·Dmitry A VasilenkoElena B Averina
Jun 17, 2020·Journal of Medicinal Chemistry·Alexandre BancetIsabelle Krimm
Mar 21, 2019·ACS Medicinal Chemistry Letters·Saara LaulumaaJette Sandholm Kastrup
Jan 9, 2020·Journal of Medicinal Chemistry·Daniel A ErlansonPaul N Mortenson

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