Enhancing catalytic stability and cadaverine tolerance by whole-cell immobilization and the addition of cell protectant during cadaverine production

Applied Microbiology and Biotechnology
Guoguang WeiPingkai Ouyang

Abstract

A whole-cell (cadaverine-producing strain, Escherichia coli AST3) immobilization method was developed for improving catalytic activity and cadaverine tolerance during cadaverine production. Cell-immobilized beads were prepared by polyvinyl alcohol (PVA) and sodium alginate (SA) based on their advantages in biocatalyst activity recovery and mechanical strength. The following optimal immobilization conditions were established using response surface methodology: 3.62% SA, 4.71% PVA, 4.21% CaCl2, calcification, 12 h, and freezing for 16 h at - 80 °C, with a cell concentration of 0.3% (g dry cell weight (DCW) per 100 mL) of immobilized beads. After a 2-h bioconversion, the immobilized beads maintained 85% of their original biocatalyst activity, which was 1.8-fold higher than that of free cells. Furthermore, the effects of cell protectants on immobilized biocatalyst activity were examined by fed-batch bioconversion experiments. The results showed that the addition of polyvinylpyrrolidone (PVP) into the immobilized matrix effectively protected biocatalyst activity, with 95% of the relative activity remaining after the 2-h bioconversion. The performance of PVA-SA-PVP-immobilized E. coli AST3 showed continuous production of cadaverine, ...Continue Reading

References

May 18, 2001·Biochemical Engineering Journal·K -M. Khoo, Y -P. Ting
Jun 5, 2004·Journal of Microbiological Methods·David ClelandDavid Emerson
Sep 27, 2007·Bioscience, Biotechnology, and Biochemistry·Takashi MimitsukaKatsushige Yamada
Jan 20, 1992·Biotechnology and Bioengineering·A MartinsenG Skjårk-Braek
Sep 9, 2008·Biotechnology Letters·Ashwini C Poopal, R Seeta Laxman
May 16, 2009·Biotechnology Advances·J D CarballeiraJ V Sinisterra
Aug 20, 2009·Applied Biochemistry and Biotechnology·Mário A P NunesMaria H L Ribeiro
Sep 3, 2010·Biotechnology and Bioengineering·Zhi-Gang QianSang Yup Lee
May 10, 2011·Applied Microbiology and Biotechnology·Jens Schneider, Volker F Wendisch
May 14, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Charles J Hunt
Dec 6, 2011·Current Opinion in Biotechnology·Judith Becker, Christoph Wittmann
Aug 16, 2015·Applied Microbiology and Biotechnology·Lennart LeßmeierVolker F Wendisch
Sep 14, 2015·Journal of Industrial Microbiology & Biotechnology·Young Hoon OhSi Jae Park

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