Enhancing comparative rabies DNA vaccine effectiveness through glycoprotein gene modifications

Vaccine
M O V OsinubiCharles E Rupprecht

Abstract

Enhancing DNA vaccine effectiveness remains a challenge, especially if the desired goal is immunization efficacy after a single dose. The glycoprotein gene from the rabies virus Evelyn-Rokitnicki-Abelseth (ERA) strain was modified by mutation at amino acid residue 333 from arginine to glutamine. The modified and original unmodified glycoprotein genes were cloned separately and developed as DNA vaccines for immunization in mice. The intramuscular (IM) route using a single dose (100 microg) of a modified DNA vaccine showed virus neutralizing antibody induction by d30, and 80% of the mice survived a challenge in which 100% of unvaccinated controls succumbed. Similar results were obtained using a single dose (10 microg) by the intradermal (ID) route with one-tenth amount of the DNA administered. Administration of single dose of DNA vaccine with unmodified G did not result in the production of detectable levels of virus neutralizing antibody by d30. The results of the IM and the ID routes of administration were statistically significant (P<0.01). Based on these preliminary results, a modified glycoprotein gene from the ERA rabies virus strain may be an ideal candidate for DNA vaccine efficacy enhancement.

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Citations

Jul 25, 2012·Current Opinion in Infectious Diseases·Philippe Gautret, Philippe Parola
Aug 23, 2011·Expert Review of Vaccines·Mazal ElnekaveAvi-Hai Hovav
Nov 3, 2011·Expert Review of Vaccines·Xianfu WuCharles E Rupprecht
Jan 23, 2016·Expert Review of Vaccines·Charles E RupprechtHildegund Ertl
Jun 13, 2015·Journal of Immunology Research·Hetron Mweemba Munang'andu, Øystein Evensen
Feb 4, 2011·Clinical and Vaccine Immunology : CVI·William C WeldonRichard W Compans
Aug 5, 2017·Clinical and Experimental Vaccine Research·Dong-Kun YangIn-Soo Cho

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