Enhancing DNA vaccine potency by co-administration of xenogenic MHC class-I DNA.

Gene Therapy
T H KangT-C Wu

Abstract

Intramuscular administration of DNA vaccines can lead to the generation of antigen-specific immune responses through cross-priming mechanisms. We propose a strategy that is capable of leading to local inflammation and enhancing cross-priming, thus resulting in improved antigen-specific immune responses. Therefore, in this study, we evaluated the immunological responses elicited through electroporation-mediated intramuscular administration of a DNA vaccine encoding calreticulin (CRT) linked to human papillomavirus type 16 E7 (CRT-E7) in combination with DNA expressing HLA-A2 as compared with CRT-E7 DNA vaccination alone. We found that the co-administration of a DNA vaccine in conjunction with a DNA encoding a xenogenic major histocompatibility complex (MHC) molecule could significantly enhance the E7-specific CD8+ T-cell immune responses and antitumor effects against an E7-expressing tumor, TC-1, in C57BL/6 tumor-bearing mice. Furthermore, a similar enhancement in E7-specific immune responses was observed by the co-administration of CRT-E7 DNA with DNA encoding other types of xenogenic MHC class-I molecules. This strategy was also applicable to another antigenic system, ovalbumin. Further characterization of the injection site r...Continue Reading

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Citations

Jan 13, 2010·Immunologic Research·Ken LinT-C Wu
Jan 11, 2018·Human Gene Therapy·Max A ChengT-C Wu
Dec 28, 2018·Biotechnology & Genetic Engineering Reviews·Armando Tejeda-MansirPatricia Guerrero-Germán

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

CELLQuest
SigmaPlot
Living Image
Systat

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