Enhancing effect of oxygen radical scavengers on murine macrophage anticryptococcal activity through production of nitric oxide

Clinical and Experimental Immunology
M TohyamaA Saito

Abstract

We examined the roles of reactive nitrogen intermediates (RNI) and reactive oxygen intermediates (ROI) in interferon-gamma (IFN-gamma)-induced cryptococcostatic activity of murine peritoneal macrophages using N(G)-monomethyl-L-arginine (L-NMMA), a competitive inhibitor of RNI synthesis, and superoxide dismutase (SOD) and catalase, oxygen radical scavengers. IFN-gamma-activated macrophages produced nitric oxide (NO) in a dose-dependent manner, as measured by increased nitrite concentration in the culture supernatant. IFN-gamma also enhanced the suppressive effect on cryptococcal growth in a similar dose-dependent manner. The induction of killing activity and NO production by an optimal dose of IFN-gamma (100 U/ml) was virtually suppressed by 500 microM L-NMMA. These results confirmed the importance of the RNI-mediated effector mechanism in anticryptococcal activity of macrophages. SOD and catalase significantly enhanced the cryptococcostatic activity of macrophages induced by a suboptimal dose of IFN-gamma (20 U/ml). The augmenting effect of these reagents was mediated by NO, since they potentiated the production of NO by macrophages and their effects were totally blocked by L-NMMA. Our results indicate that the IFN-gamma-induce...Continue Reading

References

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Citations

Aug 28, 2009·Expert Opinion on Pharmacotherapy·Michele Ritter, David L Goldman
Jan 13, 2009·Cellular Microbiology·Elena Fernández-ArenasRosalía Diez-Orejas
Mar 15, 2003·Microbiology and Immunology·Chihomi KatoKazuko Saito
May 11, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Allison J Kutner, Adam J Friedman
Dec 24, 2002·Infection and Immunity·Kelly E WeatherbyWilliam P Lafuse
Sep 2, 2020·Infection and Immunity·Yuki SatoKazuyoshi Kawakami
Aug 2, 2020·Fungal Genetics and Biology : FG & B·Ane Wichine Acosta GarciaCharley Christian Staats
Aug 1, 1996·Antimicrobial Agents and Chemotherapy·M TohyamaA Saito

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