Enhancing effect of the 3'-untranslated region of tobacco mosaic virus RNA on protein synthesis in vitro

FEBS Letters
V V ZeyenkoAlexander S Spirin

Abstract

In order to test the enhancing effect of the 3'-terminal untranslated region (3'-UTR) of tobacco mosaic virus (TMV) RNA on protein synthesis in vitro we used a chimeric mRNA construct containing TMV 5'-UTR (omega) and firefly luciferase mRNA. The addition of the TMV 3'-UTR to the chimeric mRNA construct results in a more than 3-fold stimulation of the synthesis of the functionally active protein in the wheat germ cell-free translation system. We have demonstrated that the proper length of the TMV 3'-terminal part is important for efficient translation; elongation of the TMV tail by 160 vector-derived nucleotides fully abolishes the stimulation effect of the TMV 3'-UTR in vitro.

References

Jun 1, 1991·Analytical Biochemistry·V V GurevichS A Zozulya
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B E Roberts, B M Paterson
Jun 1, 1993·Molecular and Cellular Biology·X DanthinneM Cornelissen

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Citations

Oct 1, 1996·Plant Molecular Biology·J Fütterer, T Hohn
Nov 20, 1998·Current Opinion in Biotechnology·L JermutusA Plückthun
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Laura U GillilandRichard B Meagher
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·T J L'EcuyerA B Fulton
Mar 4, 2008·Nucleic Acids Research·Gelina S KopeinaAlexander S Spirin
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M GaleM G Katze
Jul 14, 2010·Biochemistry. Biokhimii︠a︡·N E ShirokikhA S Spirin
Nov 9, 2004·Biotechnology and Bioengineering·L A ShaloikoA S Spirin
May 8, 2007·Biochemical and Biophysical Research Communications·Alexey A KovtunAlexander S Spirin
Jun 30, 2005·Biotechnology and Bioengineering·Anatoly T GudkovAlexander S Spirin
Feb 1, 2003·The Journal of General Virology·Dana N MitzelMichele E Hardy

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