Enhancing effects of indirubin on the arsenic disulfide-induced apoptosis of human diffuse large B-cell lymphoma cells

Oncology Letters
Ling WangXin Wang

Abstract

The aim of the present study was to investigate the indirubin-enhanced effects of arsenic disulfide (As2S2) on the proliferation and apoptosis of diffuse large B-cell lymphoma (DLBCL) cells in order to identify an optimum combination therapy. The human DLBCL cells, LY1 and LY8, were treated with different concentrations of indirubin for 24, 48 and 72 h. Next, the cells were treated with 10 μM As2S2 or a combination of 10 μM As2S2 and 20 μM indirubin for 48 h. Cell proliferation inhibition was detected using cell counting kit-8 and cell apoptosis was determined using flow cytometry. The expression levels of Bcl-2, Bcl-2-associated X protein (Bax) and caspase-3 were analyzed by quantitative polymerase chain reaction (qPCR) and western blotting. The DLBCL cell viability exhibited no significant changes at 24, 48 or 72 h with increasing indirubin concentration. In addition, the apoptotic rates of the LY1 and LY8 cells demonstrated no noticeable effects at 48 h with increasing indirubin concentration. Following treatment with the combination of indirubin and As2S2, the inhibitory and apoptotic rates of the cells were notably increased compared with those of the As2S2-treated group. The qPCR results revealed that indirubin alone had ...Continue Reading

References

Sep 2, 2004·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Chen ZhangAi-Xia Guo
Apr 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sangkil NamRichard Jove
Jul 5, 2005·Bioorganic & Medicinal Chemistry Letters·Jong-Won LeeSang Kook Lee
Mar 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lan WangZhu Chen
Apr 25, 2008·Bioorganic & Medicinal Chemistry·Stefanie LibnowPeter Langer
May 3, 2008·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Thiruvanmiyoor Ravichandran Kameswaran, Ravichandran Ramanibai
Jun 19, 2008·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Feng SunYu-bin Cheng
Jun 27, 2009·Archives of Pharmacal Research·Soo-Hyun KimHyo-Jeong Kuh
Oct 29, 2009·Leukemia & Lymphoma·Amel ChebelMartine Ffrench
Feb 16, 2010·Bioorganic & Medicinal Chemistry Letters·Soo Jeong ChoiYong-Chul Kim
Aug 11, 2010·Chinese Journal of Integrative Medicine·Xiao-mei HuRou Ma
Jul 24, 2012·Biochemical and Biophysical Research Communications·Katerina A NicolaouAndreas I Constantinou
Nov 8, 2012·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Radka NedomovaMarie Jarosova

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
PCR
ubiquitination

Software Mentioned

ImageQuant Las 4000
Multi Gauge
FlowJo
SPSS

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis