Enhancing enterocyte fatty acid oxidation in mice affects glycemic control depending on dietary fat

Scientific Reports
Deepti RamachandranAbdelhak Mansouri

Abstract

Studies indicate that modulating enterocyte metabolism might affect whole body glucose homeostasis and the development of diet-induced obesity (DIO). We tested whether enhancing enterocyte fatty acid oxidation (FAO) could protect mice from DIO and impaired glycemic control. To this end, we used mice expressing a mutant form of carnitine palmitoyltransferase-1a (CPT1mt), insensitive to inhibition by malonyl-CoA, in their enterocytes (iCPT1mt) and fed them low-fat control diet (CD) or high-fat diet (HFD) chronically. CPT1mt expression led to an upregulation of FAO in the enterocytes. On CD, iCPT1mt mice had impaired glycemic control and showed concomitant activation of lipogenesis, glycolysis and gluconeogenesis in their enterocytes. On HFD, both iCPT1mt and control mice developed DIO, but iCPT1mt mice showed improved glycemic control and reduced visceral fat mass. Together these data indicate that modulating enterocyte metabolism in iCPT1mt mice affects glycemic control in a body weight-independent, but dietary fat-dependent manner.

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Citations

Feb 18, 2020·Frontiers in Physiology·Changting XiaoGary F Lewis
Nov 24, 2019·Nature Communications·Mercedes Dávalos-SalasJohn M Mariadason
Jun 15, 2020·Biochemical Pharmacology·Mercedes Dávalos-SalasMagdalene K Montgomery
Feb 13, 2019·Physiology & Behavior·Wolfgang Langhans
May 20, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alyssa S ZembroskiKimberly K Buhman
Jul 21, 2021·Annual Review of Nutrition·Alyssa S ZembroskiKimberly K Buhman

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Methods Mentioned

BETA
gastric bypass
transgenic
PCR

Software Mentioned

SPSS
GraphPad Prism

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