Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors

Frontiers in Plant Science
Pia SteinrückenSvein Rune Erga

Abstract

Microalgae with a high content of the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are of great demand for microalgae-based technologies. An Arctic strain of the diatom Attheya septentrionalis was shown in previous experiments to increase its EPA content from 3.0 to 4.6% of dry weight (DW) in the nutrient-replete exponential phase and nutrient-depleted stationary phase, respectively. In the present study, a factorial-design experiment was used, to investigate this effect in more detail and in combination with varying salinities and irradiances. A mathematical model revealed that both growth phase and salinity, alone and in combination, influenced the EPA content significantly. Maximum EPA values of 7.1% DW were obtained at a salinity of 22 and after 5 days in stationary phase, and might be related to a decreased silica content, an accumulation of storage lipids containing EPA, or both. However, growth rates were lower for low salinity (0.54 and 0.57 d-1) than high salinity (0.77 and 0.98 d-1) cultures.

References

Mar 30, 2006·Journal of Bioscience and Bioengineering·Pauline SpolaoreArsène Isambert
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Qiang HuAl Darzins
Jul 27, 2012·Microbial Cell Factories·T Catalina Adarme-VegaPeer M Schenk
Jun 29, 2013·Marine Drugs·Dulce Alves MartinsKhalid M Abu-Salah
Nov 9, 2014·Eukaryotic Cell·Roshan P Shrestha, Mark Hildebrand
Jun 27, 2015·Scientific Reports·N JavaheriJ A Kaandorp
Mar 31, 2016·Sub-cellular Biochemistry·Inna Khozin-GoldbergSammy Boussiba
Apr 1, 2009·Journal of Phycology·Sebastiaan W RampenJaap S Sinninghe Damsté

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Citations

Feb 28, 2019·Physiologia Plantarum·Otilia CheregiCornelia Spetea

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Datasets Mentioned

BETA
MH020639

Software Mentioned

GraphPad Prism
Statistics and Machine Learning Toolbox
Matlab

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