Enhancing glutathione synthesis can decrease zinc-mediated toxicity

Biological Trace Element Research
U I WaltherBurckhard Fichtl

Abstract

Zinc toxicity has been linked to cellular glutathione: A decrease in glutathione is followed by an increase in zinc-mediated toxicity. The question arises whether an increase in glutathione synthesis might decrease zinc-mediated cytotoxicity. We incubated five cell lines (hepatoma and lung-derived) with zinc chloride and 2 mmol/l N-acetyl-L-cysteine (NAC) to support glutathione synthesis. In all but one hepatic cell line, the glutathione content was increased by NAC as compared to the D-enantiomere NADC, whereas NADC did not increase GSH content as compared to not treated controls. In both alveolar epithelial cell lines, an increase in zinc tolerance was observed due to NAC as compared to NADC. In native fibroblast-like and the hepatoma cell lines, no changes in zinc tolerance were found due to NAC. In the fibroblast-like cells, zinc tolerance was increased due to NAC only after cellular glutathione had been previously decreased (by lowered cysteine concentrations in the medium). Enhancing glutathione synthesis can antagonize zinc-mediated toxicity in the alveolar epithelial cell lines, whereas some other characteristics than glutathione synthesis might be more important in other cell types. Furthermore, NAC acted as a GSH prec...Continue Reading

References

Jan 1, 1992·Biology of the Neonate·K AsayamaK Kato
Dec 1, 1991·Pflügers Archiv : European journal of physiology·B GachotP Poujeol
Feb 1, 1995·Chemico-biological Interactions·B SärnstrandA Hallberg
Feb 1, 1993·Toxicology and Applied Pharmacology·O M Steinebach, H T Wolterbeek
Jun 1, 1997·The American Journal of Forensic Medicine and Pathology·D R BennettW Y Naritoku
May 13, 1999·Immunopharmacology and Immunotoxicology·M DanfourS W Evans
Feb 1, 2000·Respiratory Medicine·B ZerahnI L Kanstrup
May 10, 2000·The Journal of Nutrition·R J Cousins, R J McMahon
Aug 31, 2001·The Journal of Biological Chemistry·J SoutourinaP Plateau
Mar 13, 2003·Redox Report : Communications in Free Radical Research·Victor VitvitskyRuma Banerjee
Mar 14, 2003·Free Radical Biology & Medicine·Rachel NealNuran Ercal
Sep 5, 2003·Journal of Muscle Research and Cell Motility·Arkadiusz OrzechowskiPiotr Ostaszewski
Feb 26, 2004·Respirology : Official Journal of the Asian Pacific Society of Respirology·Behrouz JafariDeborah A Quinn
Mar 23, 2004·Annals of the New York Academy of Sciences·Arkadiusz OrzechowskiMichal Marek Godlewskia
May 29, 2004·Current Opinion in Critical Care·Fernando Frutos-VivarAndrés Esteban
Sep 19, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lysiane RichertDominique Lasserre-Bigot
Nov 7, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·U I WaltherO Temrück

❮ Previous
Next ❯

Citations

Dec 25, 2012·Environmental Science and Pollution Research International·Khaleeq AnwarAqeel Javeed
Mar 6, 2009·Experimental Lung Research·Udo I Walther, Harald Mückter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.