Enhancing hepatic fibrosis in spontaneously hypertensive rats fed a choline-deficient diet: a follow-up report on long-term effects of oxidative stress in non-alcoholic fatty liver disease

Journal of Hepato-biliary-pancreatic Sciences
Hiroya YamamotoSusumu Tazuma

Abstract

We previously reported a model of non-alcoholic fatty liver disease (NAFLD) using spontaneously hypertensive rats (SHRs), fed a choline-deficient (CD) diet for 5 weeks, that hepatic steatosis but not fibrosis is developed through oxidative stress. To determine the relationship between hypertension and hepatic fibrosis in NAFLD, we examined whether long-term CD diet leads to hepatic fibrosis through oxidative stress. Eight-week-old male SHR and normotensive Wistar Kyoto rats (WKYs) were fed a CD diet for 5 or 20 weeks, then liver histology and hepatic expression of genes related to lipid metabolism, fibrosis, and oxidative stress were assessed. Oxidative stress was assessed by hepatic thiobarbituric acid reactive substance (TBARS) levels. After 5 weeks on CD diet, prominent hepatic steatosis and decrease in expression of genes for lipid metabolism were observed in SHRs as compared with WKYs. SHRs on a CD diet demonstrated a downregulated expression of genes for antioxidants, along with significant increases in hepatic TBARS. After 20 weeks on CD diet, SHRs demonstrated severe liver fibrosis and upregulated expressions of genes for fibrosis when compared with WKY. Hypertension precipitated hepatic steatosis, and further, acts as ...Continue Reading

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Citations

Jan 11, 2017·Oxidative Medicine and Cellular Longevity·Sha LiYibin Feng
Jan 25, 2020·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Yi-Qiang LiangNorihiro Kato
Jul 9, 2020·International Journal of Molecular Sciences·Mishghan ZehraKomal Sodhi
Feb 10, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xin-Hong FengChao-Mei Ma

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